Question

In the lab procedure, it is necessary to add a reagent to glyphosate called fluorenyl orthochloroformate, or Fmoc-Cl for shor

In the last step of the procedure, dichloromethane is added to the sample, which is shaken and then spun in a centrifuge unti

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Answer #1

Fmoc-Cl is a derivatizing agent (used to alter an analyte). It reacts with glyphosate to form another new compound which absorbs light more strongly than the initial glyphosate compound.

Option B is correct.

Fmoc-Cl is soluble in Dichloromethane while the glyphosate/Fmoc-Cl is water soluble. So dichloromethane is added to separate out Fmoc-Cl from the new compound.

Option A is correct.

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