Methylation of dUMP produces _______ . a) dCTP b) dTMP c) dCMP d) dUTP
Methylation of dUMP produces (b) dTMP
If you observe the mechanism we see that TS forms a covalent bond to the substrate dUMP through a 1,4-addition involving a cysteine nucleophile. The substrate tetrahydrofolate donates a methyl group to the alpha carbon, and reduces the new methyl on dUMP to form dTMP.
Methylation of dUMP produces _______ . a) dCTP b) dTMP c) dCMP d) dUTP
If you substituted the hydrogen atoms on the methylene moiety of the dUMP intermediate (indicated by an asterisk) for a deuterium, what kind of isotope effect would you observe. H2N H2folate H2N Inverse secondary KIE HN CH2 H HN a. HN CH2 b. Normal secondary KIE он 0 0 H c. Inverse primary KIE d. Normal primary KIE CH HN 146 "О-Р-О 0 "О-Р-О- Cys146 он dTMP Он
ton is not an energy dependent process in (B)) Falls eukaryotes 43. Histone methylation is associated with Falls gene activation gene silencing Following histones are required during transcription (A) H2A and 2B (B) H3 (C) H4 (D) All of the above (E) H2AZ (c) induction (D) All of the above (E) None of the above 35. 4. DNA methylation silences gene expression AD True (B) Fall Leucine residues in lucine zipper proteins provides h ydrophobic interaction for dimerization (A) True...
17-11. Methylation of eukaryotic DNA controls gene expression. a) Describe in words the control of methylation of DNA in eukaryotes. b) Describe in words how silencing starts with methylation.
Which of the following would prevent transcriptional activators from binding? A. Increased DNA methylation B. Increased nucleosome abundance C. BOTH (A) and (B) D. NEITHER (A) or (B)
QUESTION 5 In what way does DNA methylation typically regulate transcription? A. Represses transcription. B. Activates transcription. O C. It doesn't regulate transcription at all. D. Both A and B.
Two identical twins who showed very similar methylation patterns when they were 4 years old show very divergent patterns at age 40. This divergence is most likely due to a different alternative splicing in the two individuals Ob different levels of histone acetyltransferase in the two individuals. Oc environmental differences that they experienced as adults. O d. different genomic imprinting in the two individuals. O e differences in their experiences in utero Suppose that a certain enzyme is synthesized whenever...
What would be the expected outcome of a mutation in E.coli that prevented methylation of adenine in the sequence 5'-GATC-3'? A) loss of epigenetic markers B) Randomization of mismatch repair C) increased mutation rate D) B and C E) All of these
Alterations of chromatin of DNA structure that are stable and inheritable in offspring via DNA methylation or alteration of histone proteins is referred to as _____ changes. A. epigenetic B. sensitivity C. mutational D. genetic
Complete combustion of an alkane produces a. CO2, only (D b. H2O, only (D c. CO2 and H20 d.co and H2O
Thymidylate synthase To understand how fluorouracil works, let's first take a look at how a cancer cell divides. An enzyme called thymidylate synthase must convert deoxyuridinemonophosphate (dUMP) to deoxythymidinemonophosphate (dTMP). One of the steps in this multistep process is an elimination reaction, which is shown below. RIH Elimination 052- DNA Growth / Cancer Spreads made from DUMP Enzyme Identify the role of the nitrogen substituent shown in blue. C zor made from DUMP Enzyme C C C Identify the role...