Question

4) (5 points) What fraction of Vmax­ is observed at [S] = 5 KM?

5) (20 points) For the following data:

[S] (μM)         V0 (no inhibitor)                   V0 (2.45 μM inhibitor present)

2.1                   0.031                                       0.020

4.2                   0.06                                         0.045

13                    0.138                                       0.09

20                    0.153                                       0.13

52                    0.170                                       0.135

a) Construct a 1/v (y-axis) versus 1/[S] (x-axis) plot in the space below.

b) Is the inhibition competitive, noncompetitive, or uncompetitive?

c) Calculate KM, KMapp, Vmax, and Vmaxapp.

d) What is the binding constant (Ki) of the inhibitor?

(The concentration of the inhibitor is 2.45 μM.)

4) (5 points) What fraction of Vmax is observed at [S] =5 KM? 5) (20 points) For the following data: [S] (UM) V. (no inhibito

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Answer #1

Solution Micheal is Menton que equation... V = L V max s maximum rate... Vmas [s] km. t [s] a Criven deke & [s] = 5km. - put

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