Question

You must choose between two widely accepted, commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration. Each kit uses a different enzyme and substrate. What is the most important consideration in making your choice?

A. Shelf life of reagents

B. Speed of detection

C. Wavelengths detected by microtiter plate reader

D. Sensitivity

Which assay format is the best choice for measuring small molecules, such as drugs and small peptide hormones?

A. Latex agglutination

B. Fluorescence polarization

C. Capture ELISA

D. immunochromatographis assay

Point-of-care testing is performed most easily using:

A. homogeneous enzyme immunoassay.

B. immunofixation assay.

C. immunochromatographic assay.

D. capture ELISA.

A tissue section is fixed to a microscope slide, then incubated with a fluorescently labeled antibody that binds to an antigen expressed by cells in that tissue. The preparation is then washed and inspected with a fluorescent microscope. This technique is called:

A. direct immunofluorescence.

B. indirect immunofluorescence.

C. fluorescence polarization.

D. autofluorescence.

The signal measured in a fluorescence polarization immunoassay is the:

A. wavelength of absorbed light.

B. angle of light scattering.

C. intensity of light emission.

D. orientation of emitted light waves.

Which compound would be an appropriate label in a chemiluminescent assay?

A. Horseradish peroxidase

B. Iodine-125

C. Fluorescein

D. Ruthenium derivative

Quenching of the signal emitted by the label is of particular concern in:

A. radioimmunoassay.

B. enzyme immunoassay.

C. fluorescence polarization.

D. chemiluminescent immunoassays.

Pregnancy testing can be performed at home using:

A. capture assay.

B. immunochromatography.

C. fluorescence polarization.

D. chemiluminescence.

An antigen is fixed to a microscope slide, then incubated with patient serum. After washing, fluorescently labeled anti-human immunoglobulin is applied. After an additional wash, the slide is inspected with a fluorescent microscope. This technique is called:

A. direct immunofluorescence.

B. indirect immunofluorescence.

C. fluorescence polarization.

D. autofluorescence.

Answer 1

colorimetric enzyme immunoassay kit to measure a plasma protein that circulate at moderately high concentration. each kit uses a different enzyme and substrate

You must choose between two widely accepted , commonly used colorimetric enzyme immunoassay kits to measure a plasma protein that circulates at a moderately high concentration. Each kit uses a different enzyme and substrate. What is the most important consideration in making your choice? Ans. Do sensitivity. which small Ans - assay format is molecules, such as c. Capture ELISA. the best choice for measuring drugs and small peptide hormoned Point of care testing is performed most easily using : Ans - c. immunochromatographic assay. A tissue section is fixed to a microscope slide, then incubated with a fluorescently labeled antibody that binds to an antigen expressed by cells in that tissue. The preparation is then washed and inspected with a fluorescent microscope. This technique is called Ans: BÀ: Direct immunofluorescence. fluorescence polarization The signal measured in a immunoassay is the Ans: C- Intensity of light emission.

which compound would be an appropriate label in a chemiluminescent assay ? Ans: D. Ruthenium derivative. Quenching particular Ans - c. of the signal emitted by the label is of concern in fluorescence polarization. Pregnancy testing can be performed at home using Ans: B. immunochromatography An antigen is fixed to a microscope slide, then incubated with patient serum After washing , fuorescently labeled anti- human immunoglobulim is applied. After an additional wash, the slide is inspected with afluorescent microscope. This technique is called : Ans: B indirect immunofluorescence.