Question

Please help really desperate. how do I go about this question. what graphs do I need to make and what equations do I need to get the values?? Please help I know it's a long question but its a huge chunk of my marks and I don't want to fail

Question 3. The data below was obtained from the reaction of substrate A and substrate B to generate products P and Q. Is it possible from the data to determine the reaction mechanism and if so state what this is. Use the data to determine Vmax and the Km for both substrate A and substrate B. Your answer should include tabulated transformations of the data, the graphs used to obtain kinetics values and a brief narrative explaining how you arrived at your answers e.g. The X intercept represents value - 1/A and hence A is equal to..... M 0.1 0.125 0.17 0.25 0.5 [B] = 0.05mm 1.351 1.471 1.622 V[B] = 0.1mM 1.852 2.083 2.401 2.778 3.333 V[B] = 0.25mm 2.381 2.778 3.373 4.167 5.556 v[B] = 0.5mm 2.632 3.125 3.899 5.000 7.143 1.786 2.000 vis in micromoles/min/mg

Answer 1

Enzyme inhibitors include medicines such as antibiotics, antidepressants, diuretics drugs etc. You can create a graph to depict the data related to enzyme inhibitors. A graph can be plotted to show function of the ionic strength of the solution versus molarity of the salt. Ionic strength can be defined as one-half of the sum of the molarities of each ion of the squares of ions concentrations.

Ionic strength = ½ X sum of the molarities of each ion X squares of ions concentrations

The rate equation can be :

v = (Vmax * S)/[Km (1+i/Kic] + S(1+i/Kiu)]

Here, Ki and Kic are competitive values

Kiu is uncompetitive value

It is used to for non-linear data in simple S-v plot. It is helpful to get Vmax, Km, Kiu and Kis values. These are dependent on substrate (S) and inhibitor (i) concentrations.