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Chem 111 - Experiment #8 - Lab Report Beer's Lab states that the...
Chem 162 Experiment 2 Spectrophotometric Determination of Ion Concentration by Beer's Law Pre-Lab Questions a. A...
Chem 162 Experiment 2 Spectrophotometric Determination of Ion Concentration by Beer's Law Pre-Lab Questions a. A sample absorbs 80% of the light passed through it at a certain wavelength. What is the absorbance of the sample at this wavelength? b. What would the absorbance be at the same wavelength if the sample were diluted by half? Assume the same size sample cell was used. 2, A 0.070 M solution has an absorbance ofA = 0.689. If a 1.00 cm path...
Number 2 and 4 please, thanks! BEER'S LAW EXPERIMENT 8 POST-LAB QUESTIONS Answer the following questions....
Number 2 and 4 please, thanks!
BEER'S LAW EXPERIMENT 8 POST-LAB QUESTIONS Answer the following questions. 1. what is-for the metal complex Cu(C,N,H8),SO4 from your/ class data? 2. Using the known absorbance, pathlength, and concentration of the stock solution, calculate the extinction coefficient (e) for Cu(C,N,H),so, at numerical calculations ma Show all work for 3. Wh at was the absorbance of your unknown solution? Which concentration does this correspond to on your graph? 4. A type of food coloring has...
Thanks! BEER'S LAW EXPERIMENT S PRE-LAB QUESTIONS Fully answer these questions in your laboratory notebook before...
Thanks!
BEER'S LAW EXPERIMENT S PRE-LAB QUESTIONS Fully answer these questions in your laboratory notebook before coming to lab. work for numerical calculations. 1. The wavelength at which a substance absorbs the most light is called the The metal complex Cu(C,N,H)SO, has a purple (violet) color. What would you guess as the value 2. If a solution sample has an extinction coefficient of 7.53 L mol cm,a pathlength of 1.50 cm, and an absorbance of 0.408, what is the concentration...
Lab Report: Determination of Kc for a Complex Ion Formation tube 2.00e-3 Fe3+ (mL) 2.00E-3M SCN-...
Lab Report: Determination of Kc for a Complex Ion Formation
tube
2.00e-3 Fe3+ (mL)
2.00E-3M SCN- (mL)
water (mL)
initial conc. Fe3+
initial conc. SCN-
1
5.00
5.00
0
1.00e-3M
1.00E-3M
2
5.00
4.00
1.00
1.00E-3M
8.00E-3M
3
5.00
3.00
2.00
1.00E-3,
6.00E-3M
4
5.00
2.00
3.00
1.00E-3M
4.00E-3M
5
5.00
1.00
4.00
1.00E-3M
2.00E-3M
10ml of 0.200M Fe3+, 2.00ml of 0.00200M SCN-, AND 8.00ml of
water results in an eq. [FeSCN2+] IN Standard
Soln.:2.00E-4M
Could you please explain how...
question one question two Aspirin lab (10 points) 5. a) Spectroscopy is being used to determine...
question one
question two
Aspirin lab (10 points) 5. a) Spectroscopy is being used to determine the concentration of aspirin in an unknown sample. The absorbance spectrum of the highest concentration standard [Fe3+, SA) prepared is given below. What wavelength should be used to create the calibration curve for the experiment? Explain your reasoning. 0.14 Absorbance 0.02 250 300 450 500 350 400 Wavelength (nm) Wavelength Explain your reason briefly. b) In this experiment an aspirin tablet was placed in...
I forgot to put down that the observance (=OD) at 280nm is 0.679A Experiment 8: Bradford...
I forgot to put down that the observance (=OD) at 280nm is
0.679A
Experiment 8: Bradford assay and UV-method for determination of protein concentrations Materials - An ice bucket with ice, your purified sample, three visible plastic cuvettes for Bradford assay, one UV plastic cuvette for UV-method, a piece of parafilm, a Vis-spec, a Lab Quest, Bradford reagent, white tape (share), a sharpie. Bradford assay (reference 21) 1. Add 990 uk Bradford assay reagent to a plastic cuvette 2. Blank...
Please help I am completely confused, I included the 2 graphs needed for this post lab....
Please help I am completely confused, I included the 2
graphs needed for this post lab. Thank you!
Postlab Assignments . (a) Using your own standard curve, the line equation you generated and Beer's law equation Equation 1) calculate the molar absorpivity (e) of blue dye. For all your measurem cuvette used is 1.00 cm. Use this as the value of b in Equation 1 ents, the size of (b) Using Beer's law equation again, what does a high value...
values used for table 2 how do you do the Ice tables??? Experiment V. Equilibrium Constant...
values used for table 2
how do you do the Ice tables???
Experiment V. Equilibrium Constant Lab Report ( 50 pts) Calibration Curve 1. Record the absorbance for each of the standard solutions in the table below. Calculate the concentration of FeSCN)" in each of the standard solutions and record the values in the table below. Show a sample calculation (for A2) of your work in the space below the table. Solution Absorbance [Fe(SCN)" A-1 A-2 A-3 0.000 0.390 0.535...
3. Use the below tables to complete lab calculations on the worksheet on pages 2-6 of...
3. Use the below tables to complete lab calculations on the worksheet on pages 2-6 of this document. You will submit the worksheet via dropbox on Canvas by you assigned lab time the week of March 30th through April 3rd. A document with sample calculations of different concentrations is provided to you on canvas. Enzyme Kinetics Lab Buffer volume (mL) Enzyme Volume (ml) Substrate Volume (ml) TOTAL VOLUME (mL) 0.04 0.5 1.5 0.04 0.25 1.5 0.04 0.1 1.5 0.04 0.05...
EXPERIMENT 9 CHEMICAL REACTIONS: STOICHIOMETRY LABORATORY REPORT Your Name TA's Name You must rea...
EXPERIMENT 9 CHEMICAL REACTIONS: STOICHIOMETRY LABORATORY REPORT Your Name TA's Name You must read pages 69 and 70 to complete the following calculations. Lab Section 1411. Date Trial #1 Trial #2 Trial #3 vol . 0.100 M K2CO, solution -1200 mL 12.00 mL mmole K2CrO vol. 0.100 M Pb(NO,), solution 10.00ml mmole Pb(NO) 12.00m 14.00 mL 20 g mass product+paper 0.579 g0.654 g mass dry filter paper 0266 mass PbCrO, collected precip. 9 mmole Pbcro, CALCULATIONS AND QUESTIONS (USE THE...
Chem 162 Experiment 2 Spectrophotometric Determination of Ion Concentration by Beer's Law Pre-Lab Questions a. A sample absorbs 80% of the light passed through it at a certain wavelength. What is the absorbance of the sample at this wavelength? b. What would the absorbance be at the same wavelength if the sample were diluted by half? Assume the same size sample cell was used. 2, A 0.070 M solution has an absorbance ofA = 0.689. If a 1.00 cm path...
Number 2 and 4 please, thanks!
BEER'S LAW EXPERIMENT 8 POST-LAB QUESTIONS Answer the following questions. 1. what is-for the metal complex Cu(C,N,H8),SO4 from your/ class data? 2. Using the known absorbance, pathlength, and concentration of the stock solution, calculate the extinction coefficient (e) for Cu(C,N,H),so, at numerical calculations ma Show all work for 3. Wh at was the absorbance of your unknown solution? Which concentration does this correspond to on your graph? 4. A type of food coloring has...
Thanks!
BEER'S LAW EXPERIMENT S PRE-LAB QUESTIONS Fully answer these questions in your laboratory notebook before coming to lab. work for numerical calculations. 1. The wavelength at which a substance absorbs the most light is called the The metal complex Cu(C,N,H)SO, has a purple (violet) color. What would you guess as the value 2. If a solution sample has an extinction coefficient of 7.53 L mol cm,a pathlength of 1.50 cm, and an absorbance of 0.408, what is the concentration...
Lab Report: Determination of Kc for a Complex Ion Formation
tube
2.00e-3 Fe3+ (mL)
2.00E-3M SCN- (mL)
water (mL)
initial conc. Fe3+
initial conc. SCN-
1
5.00
5.00
0
1.00e-3M
1.00E-3M
2
5.00
4.00
1.00
1.00E-3M
8.00E-3M
3
5.00
3.00
2.00
1.00E-3,
6.00E-3M
4
5.00
2.00
3.00
1.00E-3M
4.00E-3M
5
5.00
1.00
4.00
1.00E-3M
2.00E-3M
10ml of 0.200M Fe3+, 2.00ml of 0.00200M SCN-, AND 8.00ml of
water results in an eq. [FeSCN2+] IN Standard
Soln.:2.00E-4M
Could you please explain how...
question one
question two
Aspirin lab (10 points) 5. a) Spectroscopy is being used to determine the concentration of aspirin in an unknown sample. The absorbance spectrum of the highest concentration standard [Fe3+, SA) prepared is given below. What wavelength should be used to create the calibration curve for the experiment? Explain your reasoning. 0.14 Absorbance 0.02 250 300 450 500 350 400 Wavelength (nm) Wavelength Explain your reason briefly. b) In this experiment an aspirin tablet was placed in...
I forgot to put down that the observance (=OD) at 280nm is
0.679A
Experiment 8: Bradford assay and UV-method for determination of protein concentrations Materials - An ice bucket with ice, your purified sample, three visible plastic cuvettes for Bradford assay, one UV plastic cuvette for UV-method, a piece of parafilm, a Vis-spec, a Lab Quest, Bradford reagent, white tape (share), a sharpie. Bradford assay (reference 21) 1. Add 990 uk Bradford assay reagent to a plastic cuvette 2. Blank...
Please help I am completely confused, I included the 2
graphs needed for this post lab. Thank you!
Postlab Assignments . (a) Using your own standard curve, the line equation you generated and Beer's law equation Equation 1) calculate the molar absorpivity (e) of blue dye. For all your measurem cuvette used is 1.00 cm. Use this as the value of b in Equation 1 ents, the size of (b) Using Beer's law equation again, what does a high value...
values used for table 2
how do you do the Ice tables???
Experiment V. Equilibrium Constant Lab Report ( 50 pts) Calibration Curve 1. Record the absorbance for each of the standard solutions in the table below. Calculate the concentration of FeSCN)" in each of the standard solutions and record the values in the table below. Show a sample calculation (for A2) of your work in the space below the table. Solution Absorbance [Fe(SCN)" A-1 A-2 A-3 0.000 0.390 0.535...
3. Use the below tables to complete lab calculations on the worksheet on pages 2-6 of this document. You will submit the worksheet via dropbox on Canvas by you assigned lab time the week of March 30th through April 3rd. A document with sample calculations of different concentrations is provided to you on canvas. Enzyme Kinetics Lab Buffer volume (mL) Enzyme Volume (ml) Substrate Volume (ml) TOTAL VOLUME (mL) 0.04 0.5 1.5 0.04 0.25 1.5 0.04 0.1 1.5 0.04 0.05...
EXPERIMENT 9 CHEMICAL REACTIONS: STOICHIOMETRY LABORATORY REPORT Your Name TA's Name You must read pages 69 and 70 to complete the following calculations. Lab Section 1411. Date Trial #1 Trial #2 Trial #3 vol . 0.100 M K2CO, solution -1200 mL 12.00 mL mmole K2CrO vol. 0.100 M Pb(NO,), solution 10.00ml mmole Pb(NO) 12.00m 14.00 mL 20 g mass product+paper 0.579 g0.654 g mass dry filter paper 0266 mass PbCrO, collected precip. 9 mmole Pbcro, CALCULATIONS AND QUESTIONS (USE THE...
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