Question

is the product formed from the acid hydrolysis of a simple amide? 1. What is the product a. acid & base b. aldehyde & alcohol c. acid & amine d. ester & alcohol e. amine & aldehyde 2. Insulin Insulin is a polypeptide hormone that contains two short polypeptide chains linked by two interstrand disulfide bonds The most logical order of events to perform in order to sequence this protein would be: The peptides are reduced with mercaptoethanol. B. The peptides are sequenced using Edman chemistry. The peptides are separated by chromatography techniques. a. A,C,B b. C, A, B c. C, B, A d. A,B,C e. B,C,D is specific in hydrolyzing only peptide bonds in which the carboxyl function is contributed by an arginine or a lysine residue. a. Chymotrypsin b. Carboxypeptidase c. Trypsin d. CNBr e. None of the above. 4. In the SDS-PAGE (sodium dodecylsulfate - polyacrylamide gel electrophoresis) method, separation takes place on the basis of a. charge only, because all particles have different charges, but the same mass. b. the sieving action of the gel, because all particles have the same charge, but different masses. c. the sieving action of the gel, because all particles have approximately the same charge/mass ratio, but different masses. d. the chemical nature of the buffer used as the electrolyte. 5. If a protein with the sequence PQRKYPIG is treated with trypsin, what will the products be? a. PQR KYPIG b. PQRK YPIG c. PQR KYPIG d. PQ R KPIGO 6. Which of the following groups is not present in a phosphatidic acid? a. Choline forming Powered by Cognero.

Answer 1

Answer 1: c. acid and amine

Explanation: Hydrolysis under acidic conditions requires strong acids such as sulfuric or hydrochloric, and temperatures of about 100o100o for several hours. The mechanism involves protonation of the amide on oxygen followed by the attack of water on the carbonyl carbon. The tetrahedral intermediate formed dissociates ultimately to the carboxylic acid and the ammonium salt.