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Why is the Correct Order of the draw in specimen collection so important when collecting blood samples?
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Answer #1

Blood samples must be drawn specific order to avoid cross-contamination of the sample by additives found in different collection tubes.

The draw order for specimen tubes is as follows:

  1. Blood culture tube or bottle
  2. Sodium citrate tube (eg, blue top)
  3. Serum tubes, including those with clot activator and gels (eg, red, red-speckled, gold tops)
  4. Heparin tube with or without gel (eg, dark green, light green, speckled green tops)
  5. EDTA tube with or without gel separator (eg, lavender, pearl, pink tops)
  6. Sodium fluoride/potassium oxalate glycolytic inhibitor (eg, grey top)
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