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Ty should reference samples be spotted onto each plate? Fall 3. Explain how you could use TLC to monitor the course of a reac
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2. The literature Rf value standards cannot be used every time. Mobile phase, plate width & height can be changed. Solvent front and origin all these factors will not give exact reference Rf value also. Hence It is easy to identify the product spot on plate.

3. Periodically in a specific time interval starting compound (SM), Reaction mixture (RM) will be monitored by TLC, so that SM spot intensity gradually decreases and a new suspected product spot intensity will increase. This indicated reaction is under progress. In this way we can monitor the course of the reaction progress.

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