2. The literature Rf value standards cannot be used every time. Mobile phase, plate width & height can be changed. Solvent front and origin all these factors will not give exact reference Rf value also. Hence It is easy to identify the product spot on plate.
3. Periodically in a specific time interval starting compound (SM), Reaction mixture (RM) will be monitored by TLC, so that SM spot intensity gradually decreases and a new suspected product spot intensity will increase. This indicated reaction is under progress. In this way we can monitor the course of the reaction progress.
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Ty should reference samples be spotted onto each plate? Fall 3. Explain how you could use...
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The three compounds below were spotted on a TLC plate and developed to give the TLC plate shown. Which compound corresponds to which spot? Calculate the Rf value for spot B in the TLC plate shown above. When using polarity to determine the relative rates at which organic compounds travel up the TLC plate, we are considering compounds of similar molecular weight. It should also be noted that Rf values...
If standard solutions of E-stilbene and stilbene dibromide were made available to you,explain how you could use TLC to determine the identity and purity of your product.Also use your understanding of intermolecular forces to predict how stilbene dibromidewould move on a TLC plate relative to E-stilbene.
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Questions for the Thin Layer Chromatography Laboratory 1. Why can't you use a pen to mark the origin of a TLC plate? Be explicit in your answer. 2. You were impatient to see how long it would take for your sample to resolve on the TLC plate, so you constantly removed the top from the chamber. Please explain why this is an incorrect practice. 3. What happens when...
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Explain how you used TLC analysis to identify each product obtained from the dihydroxylation reactions. Explain why 100% ethyl acetate was used to develop your TLC plate (and not 100% hexane or a mixture of hexane/ethyl acetate)?
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Place approximately 10 mg of each of the four reference compounds in labeled vials or 10 X 75-mm test tubes and dissolve the samples in a few drops of methanol. In a fifth tube place a quarter of an analgesic tablet and add 1 mL of methanol. Crush the tablet with a rod, stir well, and allow the insoluble material to settle. Note that aspirin is not stable in methanol, so fresh solutions...
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Given the reaction below, consider how you could use IR
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correct instruction(s).
Help with synthesis of Lumino!!
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