Question

which wavelength is used to generate the calibration curve in the kinetics experiment in nm?

Answer 1

Question: Which wavelength is used to generate the calibration curve in the kinetics experiment in nm?

Answer:

Step 1:

To make a calibration curve, collect the UV-Vis spectrum of a variety of different concentration samples.

Step 2:

Spectrometers are often limited in linear range and will not be able to measure an absorbance value greater than 1.5.

Step 3:

Therefore, the wavelength used to generate the calibration curve in the Kinetics experiment is near UV ranging from 100-400 nm and the visible spectrum from 400-700 nm.

Near UV ranging from 100-400 nm and  visible spectrum from 400-700 nm, wavelength is used to generate the calibration curve in the kinetics experiment.