Which wavelength is used to generate the calibration curve in the Kinetics experiment? nm
Which wavelength is used to generate the calibration curve in the Kinetics experiment? nm
Homework Answers
To make a calibration curve, collect the UV-Vis spectrum of a variety of different concentration samples. Spectrometers are often limited in linear range and will not be able to measure an absorbance value greater than 1.5.
Therefore, the wavelength used to generate the calibration curve in the Kinetics experiment is near UV ranging from 100-400 nm and the visible spectrum from 400-700 nm.
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Which wavelength is used to generate the calibration curve in the Kinetics experiment? nm
which wavelength is used to generate the calibration curve in the kinetics experiment in nm?
which wavelength is used to generate the calibration curve in the kinetics experiment in nm?
Which wavelength is used to generate the calibration curve in the Kinetics experiment?
Which wavelength is used to generate the calibration curve in the Kinetics experiment?
Spectrophotometry help!? Would measuring the absorbance of the standard solutions used to prepare a calibration curve...
Spectrophotometry help!? Would measuring the absorbance of the standard solutions used to prepare a calibration curve and the unknown sample using a wavelength 10 nm higher or lower than lambda max provide an accurate concentration value for the unknown? Explain. If in an experiment, the pathlength of the light is 1 cm, and the concentration unit of the standard solutions is reported as mM, what would the units of the molar extinction coefficient, ε, be?
Measured Wavelength for Hg vapor > 900 3. A student used a Measured Wavelength (nm) vs....
Measured Wavelength for Hg vapor
> 900 3. A student used a Measured Wavelength (nm) vs. Actual Wavelength (nm) for Hg Vapor spectroscope to view the emission spectrum of Hg vapor, and plotted her measured wavelengths ly- axis) vs. the actual wavelengths (x-axis). The actual wavelength of the most prominent line in the emission spectrum of Na is 589 nm. If the spectroscope used to prepare the calibration curve is used to view the emission spectrum of Na, what wavelength...
The data in the table are used to create a calibration curve for the determination of...
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. RNA (g) 0.00 11.35 22.70 34.05 45.40 Absorbance at 260 nm 0.225 0.551 0.787 1.140 1.460 Using the slope and intercept of the...
The data in the table are used to create a calibration curve for the determination of...
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. RNA (μg Absorbance at 260 nm 0.00 0.232 10.63 0.535 21.26 0.808 31.89 1.077 42.52 1.355 m= ? in μg−1 b= Using the...
Below is question 2. Using the calibration curve equation generated in step 2, calculate the wavelength...
Below is question 2.
Using the calibration curve equation generated in step 2, calculate the wavelength (in nm) of the four hydrogen lines. Report your answers to four significant digits. 4. Scale Reading, m 2.21 5.48 12.51 35.31 Wavelength, nm D-4A D-4B D-4C D-4D
In an 'Enzyme Kinetics' experiment, the following calibration data, product (p-nitrophenol, PNP) concentration (M) versus UV...
In an 'Enzyme Kinetics' experiment, the following calibration data, product (p-nitrophenol, PNP) concentration (M) versus UV Absorbance 405 nm, was obtained If the UV absorbance 405 nm within the 1.5 mL-cuvette was measured as 0.144 in one of the trials during the experiment, determine the amount of pNP (in mg) produced. Assume total volume of reaction mixture stays at 1.5 mL at all times. (Round up to two decimal places) Molecular Weight of PNP: 139.11 g/mol PNP Concentration UV Absorbancem...
Light of wavelength 500 nm is used in a two slit interference experiment, and a fringe...
Light of wavelength 500 nm is used in a two slit interference experiment, and a fringe pattern is observed on a screen. When light of wavelength 650 nm is used a) the position of the second bright fringe is larger b) the position of the second bright fringe is smaller c) the position of the second bright fringe does not change
a) X-rays with a wavelength of 0.0045 nm are used in a Compton scattering experiment. If...
a) X-rays with a wavelength of 0.0045 nm are used in a Compton scattering experiment. If the x-rays are Compton scattered through an angle of 160°, what is the wavelength of the scattered photon? (b) A photon with an energy of 10.0 keV is Compton scattered by a free electron, which recoils with a kinetic energy of 0.200 keV. (i) What is the wavelength of the scattered photon? (ii) At what angle is the photon scattered? (think about how many...
Measured Wavelength for Hg vapor
> 900 3. A student used a Measured Wavelength (nm) vs. Actual Wavelength (nm) for Hg Vapor spectroscope to view the emission spectrum of Hg vapor, and plotted her measured wavelengths ly- axis) vs. the actual wavelengths (x-axis). The actual wavelength of the most prominent line in the emission spectrum of Na is 589 nm. If the spectroscope used to prepare the calibration curve is used to view the emission spectrum of Na, what wavelength...
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. RNA (g) 0.00 11.35 22.70 34.05 45.40 Absorbance at 260 nm 0.225 0.551 0.787 1.140 1.460 Using the slope and intercept of the...
Below is question 2.
Using the calibration curve equation generated in step 2, calculate the wavelength (in nm) of the four hydrogen lines. Report your answers to four significant digits. 4. Scale Reading, m 2.21 5.48 12.51 35.31 Wavelength, nm D-4A D-4B D-4C D-4D
In an 'Enzyme Kinetics' experiment, the following calibration data, product (p-nitrophenol, PNP) concentration (M) versus UV Absorbance 405 nm, was obtained If the UV absorbance 405 nm within the 1.5 mL-cuvette was measured as 0.144 in one of the trials during the experiment, determine the amount of pNP (in mg) produced. Assume total volume of reaction mixture stays at 1.5 mL at all times. (Round up to two decimal places) Molecular Weight of PNP: 139.11 g/mol PNP Concentration UV Absorbancem...
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