Question

The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm.

Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve.

RNA (μg	Absorbance at 260 nm
0.00	0.232
10.63	0.535
21.26	0.808
31.89	1.077
42.52	1.355

m= ? in μg−1

b=

Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.652.

mass = ? in μg

Answer 1