The data in the table at the right show a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and intercept of the best straight line fit to the calibration curve. (Do not subtract the blank reading when creating your calibration curve.)
Homework Answers
Add Answer to:
The data in the table at the right show a calibration curve for
the determination of...
The data in the table are used to create a calibration curve for the determination of...
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. RNA (g) 0.00 11.35 22.70 34.05 45.40 Absorbance at 260 nm 0.225 0.551 0.787 1.140 1.460 Using the slope and intercept of the...
The data in the table are used to create a calibration curve for the determination of...
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. RNA (μg Absorbance at 260 nm 0.00 0.232 10.63 0.535 21.26 0.808 31.89 1.077 42.52 1.355 m= ? in μg−1 b= Using the...
Calibration data for an electrochemical determination of glucose are given in the table. Glucose Concentration Current...
Calibration data for an electrochemical determination of glucose are given in the table. Glucose Concentration Current Glucose Concentration (ug/mL) (A) Current (ug/mL.) 0.00813 (nA) 0.416 3.01 107 0.0331 1.55 9.82 324 0.109 4.74 33.9 1020 0.267 11.0 123 3470 0.992 37.2 363 9550 Because these data span six orders of magnitude, most of the points will be near the origin if plotted on a lincar graph. A logarithmic plot overcomes this problem, and leads to a more even distribution of...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
The absorbance at 470 nm of the Fe(II)-phenanthroline complex was measured for several different Fe 2...
The absorbance at 470 nm of the Fe(II)-phenanthroline complex was measured for several different Fe 2 + concentrations to create a calibration curve. The absorbance at each Fe 2 + concentration was measured five times. The absorbance values, already corrected for the blank absorbance, are shown in the table. [Fe2+Fe2+] (μM) A1A1 A2A2 A3A3 A4A4 A5A5 average yiyi (yi−¯y)2(yi−y¯)2 (xi−¯x)2(xi−x¯)2 75.9 0.582 0.591 0.587 0.583 0.588 0.5862 0.0585 919.6 53.5 0.412 0.411 0.421 0.414 0.418 0.4152 0.0050 62.8 37.7 0.264...
Need help finding equilibrium molarity Table 1: Calibration Curve Data (4 pts. total) Tube Vol. of...
Need help finding equilibrium molarity
Table 1: Calibration Curve Data (4 pts. total) Tube Vol. of 0.200M Volume of Volume of Total Vol. Fe(NO3)3 0.00200M KSCN 0.500M HNO; Smi 10.5 mL 150 mL zob 20.5ml 19.98m2 J 2 5.00mL lll ML 14.05 mL 20.16 mil 5.00 ml 1.5 mL 13.5 mL 20 ml 4 5.00mL 2.0 mL 13.01 mL 20.01 mL 15 500mL I 2.50 ML 12.5 mL 20 ml Table 2: Calibration Curve Calculations and Percent Transmittance Recorded (1/2...
The data in the table are used to create a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and y-intercept of the best straight line fit to the calibration curve. Do not subtract the blank reading when creating the calibration curve. RNA (g) 0.00 11.35 22.70 34.05 45.40 Absorbance at 260 nm 0.225 0.551 0.787 1.140 1.460 Using the slope and intercept of the...
Calibration data for an electrochemical determination of glucose are given in the table. Glucose Concentration Current Glucose Concentration (ug/mL) (A) Current (ug/mL.) 0.00813 (nA) 0.416 3.01 107 0.0331 1.55 9.82 324 0.109 4.74 33.9 1020 0.267 11.0 123 3470 0.992 37.2 363 9550 Because these data span six orders of magnitude, most of the points will be near the origin if plotted on a lincar graph. A logarithmic plot overcomes this problem, and leads to a more even distribution of...
PROCEDURE Table 4.1: Dilutions of Albumin for Standard Curve Determination. SAMPLE NUMBER VOLUME OF 0.9% NACL (mL) VOLUME OF 10 mg/ml ALBUMIN STOCK SOLUTION (mL) VOLUME OF BIURET REAGENT (mL) TOTAL VOLUME IN EACH TUBE ABSORBANCE READING ALBUMIN CONCENTRATION IN SOLUTION 1 (Blank) 1.0 mL OmL 4.0 mL 0.8 mL 0.2 mL 4.0 mL .4 0.6 mL 0.4 mL 4.0 mL 5 mL 5ml sme 5mL 5ml 5mL .228 .088 1 0.271 773 1.444 11827 0.4 mL 0.6 mL 4.0...
Need help finding equilibrium molarity
Table 1: Calibration Curve Data (4 pts. total) Tube Vol. of 0.200M Volume of Volume of Total Vol. Fe(NO3)3 0.00200M KSCN 0.500M HNO; Smi 10.5 mL 150 mL zob 20.5ml 19.98m2 J 2 5.00mL lll ML 14.05 mL 20.16 mil 5.00 ml 1.5 mL 13.5 mL 20 ml 4 5.00mL 2.0 mL 13.01 mL 20.01 mL 15 500mL I 2.50 ML 12.5 mL 20 ml Table 2: Calibration Curve Calculations and Percent Transmittance Recorded (1/2...
Most questions answered within 3 hours.
-
The following are the Jensen Corporation's unit costs of making
and selling an item at a...
asked 22 minutes ago -
Does direct Medicare reimbursement of Advanced practice nurses
increase access to their services?
asked 1 hour ago -
List and explain why a company would choose to use a
published
compensation survey vs. creating...
asked 1 hour ago -
A discrete random variable X can take values from 1 to 10. Find
the variance of...
asked 1 hour ago -
The primary financial goal of a corporation is to maximize:
shareholders wealth.
earnings per share.
stock...
asked 1 hour ago -
determine whether the vectors u=(1,2,3,), v=(-2,1,0) and
w=(1,0,1) are linearly dependent or independent.
asked 1 hour ago -
python
Define a function called print_values which takes a dictionary
object as a parameter. The function...
asked 2 hours ago -
In Chapter 1 you created a program named Triangle in
which you displayed a seven-line triangle...
asked 2 hours ago -
Research question: What are the differences between separately
stated and non separately stated transactions in an...
asked 3 hours ago -
By using Arduino write a code that connects two LEDs to two
push-buttons. Each button controls...
asked 4 hours ago -
Bank of America has bonds that pay a coupon interest rate of 5.5
percent and mature...
asked 4 hours ago -
Problem: Patient Fees C++
You are to write a program that computes a patient’s bill for...
asked 6 hours ago