Question

Describe the sensitized latex particle used in the strep grouping test.
cited

Answer 1

In latex agglutination test, the antibody is adsorbed on the latex or gelatin and when the serum sample of the infected patient is added aggultination occurs (antigen-antibody) interaction.
The sensitized latex is prepared by allowing the mouse or rabbit to be sensitized to a particular antigen. The antibodies are produced by the organism is separated by affinity chromatography and the isolated antibody is covalently coupled with the latex particles.

The Strepto Grouping latex test is used for the diagnosis of streptococcal infections. The sensitized latex antibodies that are specific for the streptococci antigen is produced and when the patient containing the streptococci antigen is added, it results in agglutination indicating the presence of streptococci infection. When there is no agglutination, the result is negative and the absence of streptococci. Sensitized latex has high sensitivity and specificity for the particular antigen and it has high diagnostic accuracy.

Preparation of Streptococa- Sensitized Latex Particles Infect with Steptococce 2, House Production of antibodies in response to confections separation of antibodies by affinity chromatography Covalently couple IgG to the latex particle

Test for Steeptococe. Latex Agglutination + Streptococci Antigen .. Sentizided Latex particles Agglutination (positive)

There are different groups of streptococcal namely A, B, C, F, and G, that varies on the M-protein that are found on the cell surface of the bacteria. Sensitized latex is produced against each specific antigen and thus it helps in identifying the type of the bacterial group.