Question

1- what is the Mantoux test? what is it used to diagnose? How is it performed? explain the principle of the test in detail?

2- Name one structure or organelle that is found in BOTH eukaryotic and prokaryotic cells.

B) What is the function of that structure or organelle?

C) Describe how that structure is different between eukaryotic and prokaryotic cells.

Answer 1

Qn1.

Mantouse test is a method for Screening for tuberculosis. Also referred as tuberculin skin test. nosis of tuberculosis. Used in diagnosis Tuberculin is a sterile extract purified protein derivative derived from cultures of Mituberculosis. erumi METHOD: A little amount of tuberculin is injected under skin, of the patients forearm A small bump will form at the site of infection. If the paitent have been infected with Mel, his skin arround the site of injection should swell and harden after 28 to 72 hours. The size of the bump used to determine the result. The bump of less than 5mm is Considered as negative.

or PRINCIPLE: Man touse test is based on on Type W delayed hypersensitivity reaction. This test used to check individuals cell mediated immunity agaist. the pathogen Mycobacterium tuberculosis After tuberculin injection, cytokines are released by memory T-cells to attract me nacrophages and which creates induration at the area of injection, in case of Mycobacterium tuberculosis ex ese posed person. granulocyte

Qn2.

Ribosome found both in eukaryotes and prokaryotes.

B.

Ribosome is made up of rRNA and several proteins. Their main function is protein synthesis both in eukaryotes and prokaryotes .

C.

Prokaryotic ribosome	Eukaryotic ribosome
1. Sedimentation coefficient 70S. 2. Small in size. Mass 27000kd. 3. Made up of 50S and 30S subunits. 4.Diameter is 200A°. 5. Occurs freely in cytoplasm.	1.Sedimentation coefficient 80S. 2. Large in size. Mass 42000kd. 3.Made up of 60S and 40S subunits. 4. Diameter 250-300A°. 5.Mostly found as attached to RER of nucleus membrane.

505 subunity 55 RNA 5.85 RNA 59RNA 60s subunit 235 RNA 285RNA 16S RNA 18 S RNA 303 a subunit *40s subunit 70s Prokaryotic RNA 80s Eukaryotic RNA