1.) Explain in detail how has yeast been used to analyze eukaryotic gene function 2.) Name...
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1.) Explain in detail how has yeast been used to analyze
eukaryotic gene function
2.) Name...
1. Describe the two yeast expression plasmids used to transform a strain of yeast. 2. How...
1. Describe the two yeast expression plasmids used to transform a strain of yeast. 2. How do the plasmid vectors (how do their expression products) lead to us knowing whether a known protein and unknown protein bind or interact? Questions on DNA Testing for Genetic Diseases Associated with Mutations: 3. What are the factors that lead to limits of detecting genetic diseases with DNA molecular testing? 4. What is the difference between information gathered by screening for a disease and...
1- what is the Mantoux test? what is it used to diagnose? How is it performed?...
1- what is the Mantoux test? what is it used to diagnose? How is it performed? explain the principle of the test in detail? 2- Name one structure or organelle that is found in BOTH eukaryotic and prokaryotic cells. B) What is the function of that structure or organelle? C) Describe how that structure is different between eukaryotic and prokaryotic cells.
1) Explain how eukaryotic gene expression is controlled from a distance 2) Describe the experiments that...
1) Explain how eukaryotic gene expression is controlled from a distance 2) Describe the experiments that showed all differentiated cells contain all the genetic instructions to direct the formation of a complete organism. 3) Explain the structure of tryphtophan operon and how this operon is regulated at the low and high concentration of tryphtophan.
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin?...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin? (A-E) A E പറ B D с 2. Aeromonas hydrophila, the bacterium with this plasmid, was found growing in what organism? 3. The qach and qacEA1 genes provide resistance to what group of disinfectant?? 4. The tetA and tetR genes provide resistance to what type of antibiotics? Aeromones hydrophile plasmid R18 165.906be GC 52.53% 5.. Name the type of horizontal gene transfer diagrammed below....
Question 9 1 pts You are given a flask with standard liquid media containing a population...
Question 9 1 pts You are given a flask with standard liquid media containing a population of S. pombe cells that have a temperature sensitive mutation of a gene that causes complete loss of function of the gene product at the restrictive temperature. The flask had been incubating at the permissive temperature in the dark before it was given to you, and the cells in the flask were replicating asynchronously with respect to the stage of the cell cycle. The...
Gene: sax-3 Question: Test a different neuron to see how wide spread your function is: You...
Gene: sax-3 Question: Test a different neuron to see how wide spread your function is: You just found that your protein is involved in neural development in PHB neurons. To test if this is a wide-spread effect, you can label other neurons in the animal using a transgenic array, and introduce the transgenic array into your mutant. 1. Explain the approach and why it would be useful? 2. Show Genetic Crosses You would do in C. elegans to introduce the...
Name: Cell Review Prokaryotes vs. Eukaryotes: 1. What is the most defining/distinguishing feature of a eukaryotic...
Name: Cell Review Prokaryotes vs. Eukaryotes: 1. What is the most defining/distinguishing feature of a eukaryotic cell? eeld 2. Which is bigger: eukaryotic cell OR prokaryotic cell? 3. Which type of cell has membrane-bound organelles, like a mitochondria? 4. Which type of cell has ribosomes? 5. Which type of cell has cell membranes? Quick Eukaryotic Cell Function Questions: 1. What is the endoplasmic reticulum composed of? 2. What is the largest organelle of the cell? 3. Which organelle is responsible...
2. A homolog of your favorite gene, YFG1, has been identified in S. cerevisiae. You were...
2. A homolog of your favorite gene, YFG1, has been identified in S. cerevisiae. You were tasked with: i. generating a null allele of YFG1 in a diploid strain with the genotype: his3/his3, TRP1/trp1, ura3/URA3, YFG/YFG using a one-step gene replacement. [ So after the gene replacement genotype will be his3/his3, TRP1/trp1, ura3/URA3, yfg:marker/YFG] i. Sporulating the knockout strain generated in (i) iii. dissecting the resulting tetrads (asci) iv. testing the resulting spores on a variety of growth media The...
ANSWER THE FOLLOWING QUESTIONS 1) What is the relationship between a gene and a protein? 2)...
ANSWER THE FOLLOWING QUESTIONS 1) What is the relationship between a gene and a protein? 2) How does the tRNA contribute to protein synthesis? 3) The ability of cell to control their gene expression is called 4) What is a promoter? 5) What process convert the message from mRNA into amino acids? 6) The following expressions are true or false? Explain a. "A gene is any DNA sequence that is transcribed to any type of RNA b. "In eukaryotes, a...
3. (2 points) In the nematode roundworm, Caenorhabditis elegans, the LIN-14 protein controls the timing of...
3. (2 points) In the nematode roundworm, Caenorhabditis elegans, the LIN-14 protein controls the timing of certain cell divisions during development. LIN-14 protein levels are normally high in early development but decrease in the later stages. In a lin-4 mutant, the level of LIN-14 protein stays high throughout development, changing the pattern of cell divisions in the animal and producing defects in the shape of the animal. The lin-4 gene encodes a microRNA that binds to a sequence in the...
1. Which strain of Escherichia coli has been transduced with the gene for the Shiga toxin? (A-E) A E പറ B D с 2. Aeromonas hydrophila, the bacterium with this plasmid, was found growing in what organism? 3. The qach and qacEA1 genes provide resistance to what group of disinfectant?? 4. The tetA and tetR genes provide resistance to what type of antibiotics? Aeromones hydrophile plasmid R18 165.906be GC 52.53% 5.. Name the type of horizontal gene transfer diagrammed below....
Question 9 1 pts You are given a flask with standard liquid media containing a population of S. pombe cells that have a temperature sensitive mutation of a gene that causes complete loss of function of the gene product at the restrictive temperature. The flask had been incubating at the permissive temperature in the dark before it was given to you, and the cells in the flask were replicating asynchronously with respect to the stage of the cell cycle. The...
Name: Cell Review Prokaryotes vs. Eukaryotes: 1. What is the most defining/distinguishing feature of a eukaryotic cell? eeld 2. Which is bigger: eukaryotic cell OR prokaryotic cell? 3. Which type of cell has membrane-bound organelles, like a mitochondria? 4. Which type of cell has ribosomes? 5. Which type of cell has cell membranes? Quick Eukaryotic Cell Function Questions: 1. What is the endoplasmic reticulum composed of? 2. What is the largest organelle of the cell? 3. Which organelle is responsible...
2. A homolog of your favorite gene, YFG1, has been identified in S. cerevisiae. You were tasked with: i. generating a null allele of YFG1 in a diploid strain with the genotype: his3/his3, TRP1/trp1, ura3/URA3, YFG/YFG using a one-step gene replacement. [ So after the gene replacement genotype will be his3/his3, TRP1/trp1, ura3/URA3, yfg:marker/YFG] i. Sporulating the knockout strain generated in (i) iii. dissecting the resulting tetrads (asci) iv. testing the resulting spores on a variety of growth media The...
ANSWER THE FOLLOWING QUESTIONS 1) What is the relationship between a gene and a protein? 2) How does the tRNA contribute to protein synthesis? 3) The ability of cell to control their gene expression is called 4) What is a promoter? 5) What process convert the message from mRNA into amino acids? 6) The following expressions are true or false? Explain a. "A gene is any DNA sequence that is transcribed to any type of RNA b. "In eukaryotes, a...
3. (2 points) In the nematode roundworm, Caenorhabditis elegans, the LIN-14 protein controls the timing of certain cell divisions during development. LIN-14 protein levels are normally high in early development but decrease in the later stages. In a lin-4 mutant, the level of LIN-14 protein stays high throughout development, changing the pattern of cell divisions in the animal and producing defects in the shape of the animal. The lin-4 gene encodes a microRNA that binds to a sequence in the...
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