In which direction would the following molecules move in the electric field of an electrophoresis at the indicated pH?
a) Albumin (pI = 4.9) at pH 8.0
b) Ureasa (pI = 5.0) at pH 3.0 and 9.0
c) Ribonuclease (pI = 9.5) at ph 4.5, 9.5 and 11.0
d) Pepsin ((pI = 1.0) at pH 3.0, 7.0 and 9.5
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In which direction would the following molecules move in the electric field of an electrophoresis at...
A variety of spectra for an organic compound with molecular
formula C10H16O are presented below. The
experimental accurate mass using (+) APCI source is 153.1280 u. The
1H, 13C, COSY, HSQC and HMBC NMR spectra are given in the following
slides. Propose a structure for this unknown and answer or address
the following questions or requirements:
d. If there are still any questionable assignments, propose
additional NMR experiments which would solve those questions and
briefly explain specifically what correlations you...
Boxes Task: Evaluate whether you can load various sizes of jars into boxes. For each test, you will input the length, width, and height of a box as well as the diameter and height of a cylindrical jar. The algorithm will determine whether each jar will fit in its corresponding box. The jar can sit upright in the box, or it can lie on its side with its top parallel to one of the sides of the box. Assume that...
3. Based on the integration of the peaks, what is the relative
number of protons which make up each signal?
4. Identify any common splitting patterns. (ie. Isopropyl,
ethyl, etc)
UN (43 MIL.) 10.02s, 1H), 7.716.J-2 Hz. ) 2.0 11.5 11.0 10.5 10.0 9.5 9.0 8.5 8.0 7.5 7.0 6.5 6.0 4.0 3.5 3.0 2.5 20 15 100.5 0.0 -0.5 -1.0 -1.5 -2. 5.5 5.0 4.5 fl (ppm)
х у V 1.0 7.6 1.0 1.0 2.0 8.7 11 2.0 2.0 3.0 7.3 3.0 3.0 4.0 5.8 11 10- 9- 8 7-1 6 5- 4+ 3+ 2 4.0 4.0 11 10 9+ 8+ 71 6+ 5+ 4+ 3+ 2+ 1. X 5.0 8.2 5.0 5.0 6.0 4.9 6.0 6.0 X 7.0 4.5 7.0 7.0 8.0 7.2. 8.0 8.0 0 1 2 3 4 5 6 7 8 9 10 11 7 8 9 10 11 9.0 5.9 9.0 9.0...
______ 2. Using the accompanying table, determine which protein would stick to a carboxymethyl cellulose ion exchange column (the column has stationary carboxyl groups) at pH 7.0. a) glucose oxidase b) horse myoglobin c) ribonuclease A d) cytochrome C Protein Isoelectric Point Amyloglucosidase Glucose oxidase Trypsin inhibitor β-Lactoglobulin Carboanhydrase Myoglobin horse Lentil lectin Ribonuclease A Cytochrome C pI 3.5 pI 4.2 pI 4.5 pI 5.15/5.3 pI 6.0 pI 7.35 pI 7.75/8.0/8.3 pI 9.45 pI 10.65
5. Based on this analysis, the compound might be or contain
the following substructure:
6. How many different types of carbons appear to be present?
What are the chemical shifts for these carbons?
UN (43 MIL.) 10.02s, 1H), 7.716.J-2 Hz. ) 2.0 11.5 11.0 10.5 10.0 9.5 9.0 8.5 8.0 7.5 7.0 6.5 6.0 4.0 3.5 3.0 2.5 20 15 100.5 0.0 -0.5 -1.0 -1.5 -2. 5.5 5.0 4.5 fl (ppm)
How would you analyze the peaks for a 1H NMR of
Bromovanillyl Alcohol and an IR spectra of Bromovanillyl
Alcohol?
IR Peaks
NMR Peaks
%Transmittance 3446.63 3121.86 8 3004.87 2925.69 2877.64 8 1611.72" 1588.93 1499.44 1420.46 1398.31 1371.05 05 1277.51 1200.01 1179.94 1143.10 8 1047.61 1010.21 948.66 852.87 836.91 823.94 780.44 719.71 H NMR (400 mHz, CDCls) Br 6.86 s) 92 7.09 5.90 4.60 1.64 CDCls H20 12.5 12.0 11.5 11.0 10.5 10.0 9.5 9.0 8.5 8.0 7.5 7.0 6.5...
2. For the following molecules, which method would you use to separate them? Gel filtration chromatography or Ion-exchange chromatography? A) Glucose oxidase (M.W. 160 kDa, pI 4.8) and lactate dehydrogeanse ( M.W. 140 kDa, pI 8.0) B) Glucose Oxidase ( M.W. 160 kDa, pI 4.8) and beta galactosidase (M.W. 500 kDa, pI 4.5)
Investigation 2 please
Exploring the electric field of point charges Use PHET interactive CHARGES AND FIELDS. Investigation 1: Electric field due to single charges 1. Use 3 negative -1 nC charges on top of each other to make a point charge 41 = -3.0 nC on the left(ish) side of the grid, and note this point as the origin. 41 X 06 1.0 m 5.0 m 2. Use the field sensor to measure and record the electric field Ex due...
Hi it's my code for python
I almost finished my project but only one thing left which is
most confusing part please help me
I have to find most occurring ending character from a to z
For instance, output should be like this I have to find a to
z.
Words starting with ‘a’ end mostly with ‘O’
Words starting with ‘b’ end mostly with ‘O’
......
No words start with ‘O’(If there's no word in the character from a...