Question

11. Carbonic anhyd rase of red blood cells, which has a molecular weight of 30,000 is among the most active enzymes known. It catalyzes the reversible hydration of CO2 HCO,+ H H2O+ CO2 If 10 ug of pure carbonic anhyd rase catalyzes maximum hydration of 0.30 g of carbon dioxide in 1 min. at 37°C under optimal conditions, calculate the turnover number of carbonic anhydrase

Answer 1

numkey Turn ovRY product permole I&he rales Turn-avern numbers of Catayst per unit ime oresene al So Turn num aer s 0nrer Keat as mole so co anysel peruan K mlesof catalyst Turn ver number Keat = canb mi anay drase mass a Molar catay ed tever Sibla hydraHo reaehon ot co, the Caron co3t H20 COL Cen bydruse 10 carbenic anhdrace lo xio Maly Carbonie an 30, m 3.33 x101o A2ain, o30 O30 cO 2 = 6.8166 x103 44.012me me Leco So 3-33 x16 anhydrase Conver k 6-8ix Carb on'e Cor ७ pa mnute Hen ee mole o carboni anhy drase Converts 68166x 103 20.44 10 male 3 33 X101o o per minhn aver number oCor bonade onhy drase Tun 20.47x10min 1mina o Sec) 3.4116G xI