1. The hydration of carbon dioxide CO2 in red blood cells takes place due to an enzyme catalytic process, where bicarbonate ions are formed:
CO2(g) + H2O(l) ® HCO3¯ (aq) + H+(aq)
At the enzyme concentration of [E]0 = 2.3´10-6 mol/L, the following kinetic data for initial reaction rate vs. initial concentration of carbon dioxide were obtained:
[CO2], mol/L |
1.25 |
2.50 |
5.00 |
20.00 |
Rate, (mol/L)s-1 |
2.78´10-2 |
5.01´10-2 |
8.32´10-2 |
1.67´10-1 |
Determine the Michaelis constant and the turnover number of the catalytic process.
2. The enzyme a-chymotrypsin is secreted in the pancreas of mammals and cleaves peptide bonds made between certain amino acids. Several solutions containing the small peptide N-glutaryl-1-phenylalanine-p-nitroanilide (a substrate) at different concentrations were prepared and the same amount of a-chymotrypsin was added to each one. The following data were obtained on the initial rates of the formation of product:
[S]0, mol/L |
0.334 |
0.450 |
0.667 |
1.00 |
1.33 |
1.67 |
Rate, (mol/L)s-1 |
0.152 |
0.201 |
0.269 |
0.417 |
0.505 |
0.667 |
Determine the maximum reaction rate and the Michaelis constant for the enzyme reaction.
1.
The plot of 1/ rate vs 1/ [CO2] is as shown
Y intercept
Hence, the maximum reaction rate
Slope
Hence, the Michaelis constant
1. The hydration of carbon dioxide CO2 in red blood cells takes place due to an...
The enzyme carbonic anhydrase catalyzes the hydration of CO2 gas in red blood cells: CO2 (g) + H2O (L) →H2CO3 (aq) Determine the ∆Ssys of this reaction at 37 °C (body temperature), and justify the sign. *Thermodynamic parameters at T=298K and 1 bar Sm (JK-1 mol-1) Cp (JK-1 MOL-1) CO2 (g) 213.7 36.8 H2O (L) 69.9 78.2 H2CO3 (aq) 187.4 19.4
carbon monoxide reacts with nitrogen dioxide to produce carbon dioxide and nitric oxide as given by the following reaction. CO (g) + NO2 (g) → CO2 (g) + NO (g) The reaction is zeroth order in CO and second order in NO2, and second order overall. The rate constant for this reaction at 175ºC is 7.58 × 10-4 L mol-1 s-1. If the initial concentration of NO2 was 0.565 M, what is the molar concentration of NO2 after 5.8 minutes?...
3. The carbon dioxide produce in animal cells is transported to the lungs mostly dissolved in blood plasma. Essential to this process is the reaction of CO2 with water. Given the AHE= -612.96 kJ/mol for carbonic acid, calculate the AHreaction at 298.15 K. H2O(l) + CO2(g) 2 H2CO3(aq)
Complete the following sentences describing the reactions of CO2 in plasma. red blood cells carbonic anhydrase low left reversible acidic right cytoplasm basic arrow The concentration of carbon dioxide determines the following reaction: H2O + CO2 + H2CO3 The above reaction is catalyzed by the enzyme carbonic anhydrase found in In the tissues, where CO is high, the reaction will move to the making the blood more making In the lungs where CO2 is low and H2CO3 is high, the...
2. What is the percent yield of CO2 (44.01 g/mol) if 0.275 g of carbon dioxide is obtained by heating 1.75 g of calcium carbonate, CaCO3 (100.09 g/mol)? (10 points) show all setup & work CaCO3) + CaO) + CO2Q Answer: % yield = A MULA needed to react with excess CaCO3 to produce 32.0 L of CO2 at S
The following data were obtained for the reaction of methane with oxygen: CH4(g) + 202(g) CO2(g) + 2H20(1) → time(min)[CH4l (mol/L) 0 10 20 30 0.050 0.030 0.020 0.015 0.020 a) How many moles of CO2 are produced for each mole of CH4 that is used up? b) What concentration of CH4 is used up after 10 minutes? c) What is the concentration of carbon dioxide produced after 20 minutes? d) Write an equation for reaction rate in terms of...
Nitrogen dioxide reacts with carbon monoxide to produce nitric oxide as follows: NO2 (9) + CO (9) ► NO (9) + CO2 (g) The reaction is second order in NO2, zeroth order in CO and second order overall. How long (in hours) will it take for NO2 to decompose by 35.8% given the initial concentration was 0.43 M NO2 and CO was in excess. The rate constant for this reaction at 225°C is 2.08 x 10-4 L/mol/s. Report your answer...
Acetoacetic acid, CH3COCH2COOH, decomposes in aqueous acidic solution to form acetone and carbon dioxide: CH3COCH2COOH(aq) --> CH3COCH3(aq) + CO2(g) The reaction is first order. At room temperature, the half-life of the reactant is 139 min. a. What is the rate constant of the decomposition reaction ? b. If the initial concentration of acetoacetic acid is 2.75 M, what is its concentration after 5 hours ?
18. Pure carbon dioxide (Pco2 = 1 atm) decomposes at high temperature. For the reaction system 2 CO2(9) #2 CO(g) + O2(9) the percentage of carbon dioxide that undergoes the decomposition reaction is as follows: Temperature Decomposition (K) 1500 0.048 2500 17.6 3000 54.8 Is this reaction endothermic? Calculate the value of K, at each temperature. 19. Elements of Group 16 form hydrides with the generic formula H.X. When gaseous H,X is bubbled through a solution that is strongly acidic,...
answer both parts 1 and 2 Acid precipitation dripping on limestone produces carbon dioxide by the following reaction: CaCO3(s) + 2H+ (aq) —— Ca²+ (aq) + CO2(g) +H20(1) 19.5 mL of CO2 was produced at 25.0°C and779.0 mm Hg. Part 1 (1.5 points) How many moles of CO2 were produced? mol Part 2 (1.5 points) How many milligrams of CaCO3 were consumed? mg