Question

Making solutions with multiple solutes (Part II) 0.15 molo / 17.582 9 gnad 1. (5 points) Describe how to prepare a 2L solution: 15 mol 250 mM NaCl 58.449/md Y2LX 0.15 m 1% NP-40 (v/ve) 0.01 X 26 2 9 0.1% SDS (w/v), 0.001% 2L 0.0029 / SAS 50 mM Tris-HCI 0.05 X 62.14 = 6.0579 16.057 g o Hell + DI Hgo to a I to o to 2. (4 points) Describe how you would prepare 25 mil of the following som 137 mm Naci 0.1379/md X 0.25 L X 58.44 10 M NaHPO. 268.07 x 0.25LXidmd=670.175 2 mM KH2PO4 (from a 50 mM KH2PO, stock) Mui Dave 3 3. (5 points) Describe how you would prepare 500 ml of the following 63 mM Tris-HCI "121,1491md X WA 10% Glycer ml=h 1 600 m42 lycerol (v/v)e olx ore=0.05 2% SDS (w/v) 0.0 2 x 0.52 = 0.1 0.0025% Bromophenol Blue (v/v) 0.00025 X 500ML = 0.125 5. (6 points) Calculate the amount of each solute needed to make 100 mL of the following solution 2% (w/v), Tryptone 0.02 X 160mL 0.5% (w/v) Yeast Extract 0.005 X100ML 0.05% (w/v), NaCl 0.000 5x loomL 2.5 mM KCl (from a 25 mM KCl stock) 10 M MgCl2 95.21 XL X10 = 95.21
please check my answer if i did right

Answer 1

Few aren't correct. I' ve solved all with an explanation. I hope it helps:)

solution Prepare 22 150mm Nace 150mm = 0,15 M. Molecular weight of Nace ² 58.64 g/mol grams of Nace = 2 0.15 x 58.44 x 2 17.532g of Nace for at sensin Ara b) 1 X 2000 100-1 - a 20.20g NP rojan 22 solution I In this 200ml question It'll be 2000 mL not 2 .002 q SDS -100-01 Here convert 22 into mL i.e. - 2000ml 50 mM Tris-hce Iris the MW - 157.60 g/mal som M - 0.05M. Gran of Tris. H4 = 0.05 X 157.60 x 2 = 15.76 g cef Tre's-Hee The weight of this = 121.14 almal & bis ice = 157.60 glma

A2 a 25 mL 137m M Nace Nace Mwa 58 44 glmal 137mM= 0. 137 M. Q 137 x 58.44 x 25 – 0.2 g of Noce A b? 10 M af Naz HPO4 to MW cef Na, HPO4 = 141.96 glmal - 10x 141.964 0.025 = 35.49g of Nag Hooz 2 mu, a mv2 20mm x 25 = 50m X V₂ Va do lomL. ( - A 3 A) 63mM Triste MW= 157.60 g/mal 500 ml sal of 0.063 x 157.60 x 0.5 - = 4.96 g of Tris hee 10 x 500 = 5000 = $S65 55.55g 1008-lo b) do 2000 - 10:20 g 2x 500 98 100-2

0,01259 0.0025 X 500 10o -0.0025 A5 a 2 et 2 x 100 100-2 = 200 = 2:04g 0.5x loo 100-0.5 0.5 g 6.05 x 100 100 -0.05 = 0.05 g m, via m, v2 25x10o = 250 x uz V₂ = 10mL e) 10M MgCl2 Mw of Mach a 10x 95.211 x 100 95.211 glmal & 95.2119 loop Secunoscillo