How is Km determined from an Eadie-Hofstee plot? A.) By taking the negative inverse of the...
How is Km determined from an Eadie-Hofstee plot?
A.) By taking the negative inverse of the x-intercept
B.) Km is the negative slope of the curve
C.) Km is determined by multiplying the value of the slope by Vmax
D.) Km is determined by dividing Vmax by the x-intercept
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How is Km determined from an Eadie-Hofstee plot?
A.) By taking the negative inverse of the...
The Eadie-Hofstee plot shown below, is an alternative graphical representation of Michaelis-Menten kinetics. t plots t...
The Eadie-Hofstee plot shown below, is an alternative graphical representation of Michaelis-Menten kinetics. t plots the rate (v) versus the ratio of the rate over the substrate concentration (vIS]). This plot is typically used to determine the maximum rate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the slope in terms of the constants Vmax and Km Eadie-Hofstee Plot y-intercept Slope x-intercept AS
For the Eadie-Hofstee plot described in lecture, manipulate the Michealis-Menten equation into the form used for...
For the Eadie-Hofstee plot described in lecture, manipulate the Michealis-Menten equation into the form used for Eadie-Hofstee. Then, indicate a relationship for each the slope, y-intercept, and x-intercept in terms of Vmax and/or KM
The kinetics of enzyme catalyzed reactions can be described the Michaelis-Menten equation and the Eadie-Hofstee equatio...
The kinetics of enzyme catalyzed reactions can be described the Michaelis-Menten equation and the Eadie-Hofstee equation as shown below: V0 = (-Km) V0 / [S] + Vmax a). Please derive the Eadie-Hofstee equation starting from the Michaelis-Menten equation. b). The Vmax and Km of the enzyme catalyzed reaction can be derived from a plot of V0 versus V0/[S]. Please draw one of these plots and explain how do you use it to derive Vmax and Km. c). Please draw a...
How can the Michaelis-Menten constant, be derived from this Lineweaver-Burk plot? Vmax O km = (-1)/(x-intercept)...
How can the Michaelis-Menten constant, be derived from this Lineweaver-Burk plot? Vmax O km = (-1)/(x-intercept) O km = (-1) * (x-intercept) O km = 1/(x-intercept) 0 Km = s;lope
Here is a question to be sure you are able to calculate Km and Vmax from...
Here is a question to be sure you are able to calculate Km and Vmax from axes from a Lineweaver-Burk plot. If the y-intercept of a given plot is 0.25 min/uM and the slope is 0.65 min, what are the values of Vmax and Km for this given enzyme? Please Include the work.
4. The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is give...
4. The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by: 1 Km 1 1 where, the plot of (1/V.) vs (1/[S]) is a linear plot. If you only know the x-axis and y-axis intercepts from this plot, how can you determine Vmax and Km? (A) multiply the reciprocal of the x-axis intercept by -1. (B) multiply the reciprocal of the y-axis intercept by -1. (C) take the reciprocal of the x-axis intercept. (D) take...
QUESTION 9 For an enzyme capable of using substrates M and N, a plot of 1/Vo...
QUESTION 9 For an enzyme capable of using substrates M and N, a plot of 1/Vo vs. 1/18] is made. The Y-intercept (.e. the value of Y when X = 0) is found to be the same for both substrates IM and N). This indicates that the enzyme has the same for both substrates A. Vmax B. Km C. Km and Vmax D. AG
how we can solve q2 2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant...
how we can solve q2
2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant kl) ク ES→ E + S (rate constant k2) E S ES-XⓟHE) orate constant k3) So d[PVdt- k3[ES] Use the steady state approximation to show [El/[ES] (k2+k3)/(k1[S] b) let Km=(k2+k3)/kl and show that you get the expression ·J [EVIES]-Km/[S] c) We will talk in class about how this information eventually gives rise the expression d[P]/dt-k3E][S/(Km +IS) Usually [S>>Km. Show what this equation simplifies to...
5.How would you determine the values of KM and Vmax from a: a) Michaelis-Menten plot b)...
5.How would you determine the values of KM and Vmax from a: a) Michaelis-Menten plot b) Lineweaver-Burke plot 6. How is general acid/base catalysis different from regular acid/base catalysis? 7. How would you recognize the process of covalent catalysis in an enzyme reaction mechanism?
A student determined the value of K for the reaction 2 H2 (g) + O2 (g)...
A student determined the value of K for the reaction 2 H2 (g) + O2 (g) → 2 H2O (g) at several different temperatures. The value for ∆S can be determined from: the slope of the curve at 25ºC from a plot of ln K versus T. the y-intercept of the line resulting from a plot of ln K versus T. the y-intercept of the line resulting from a plot of ln K versus (1/T). the slope of the line...
The Eadie-Hofstee plot shown below, is an alternative graphical representation of Michaelis-Menten kinetics. t plots the rate (v) versus the ratio of the rate over the substrate concentration (vIS]). This plot is typically used to determine the maximum rate, Vmax, and the Michaelis constant, Km, which can be gleaned from the intercepts and slope. Identify each intercept and the slope in terms of the constants Vmax and Km Eadie-Hofstee Plot y-intercept Slope x-intercept AS
How can the Michaelis-Menten constant, be derived from this Lineweaver-Burk plot? Vmax O km = (-1)/(x-intercept) O km = (-1) * (x-intercept) O km = 1/(x-intercept) 0 Km = s;lope
4. The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by: 1 Km 1 1 where, the plot of (1/V.) vs (1/[S]) is a linear plot. If you only know the x-axis and y-axis intercepts from this plot, how can you determine Vmax and Km? (A) multiply the reciprocal of the x-axis intercept by -1. (B) multiply the reciprocal of the y-axis intercept by -1. (C) take the reciprocal of the x-axis intercept. (D) take...
QUESTION 9 For an enzyme capable of using substrates M and N, a plot of 1/Vo vs. 1/18] is made. The Y-intercept (.e. the value of Y when X = 0) is found to be the same for both substrates IM and N). This indicates that the enzyme has the same for both substrates A. Vmax B. Km C. Km and Vmax D. AG
how we can solve q2
2. a) The Michaelis-Menten mechanism is +KTERE] - @s→Es (rateco nstant kl) ク ES→ E + S (rate constant k2) E S ES-XⓟHE) orate constant k3) So d[PVdt- k3[ES] Use the steady state approximation to show [El/[ES] (k2+k3)/(k1[S] b) let Km=(k2+k3)/kl and show that you get the expression ·J [EVIES]-Km/[S] c) We will talk in class about how this information eventually gives rise the expression d[P]/dt-k3E][S/(Km +IS) Usually [S>>Km. Show what this equation simplifies to...
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