Here is a question to be sure you are able to calculate Km and Vmax from...
Here is a question to be sure you are able to calculate Km and Vmax from axes from a Lineweaver-Burk plot. If the y-intercept of a given plot is 0.25 min/uM and the slope is 0.65 min, what are the values of Vmax and Km for this given enzyme? Please Include the work.
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Here is a question to be sure you are able to calculate Km and
Vmax from...
Name Page Number Date Vmax 1. Estimate Vmax and Km using the velocity vs. substrate concentration...
Name Page Number Date Vmax 1. Estimate Vmax and Km using the velocity vs. substrate concentration plot - KM 2. Calculate the Vmax and Km using the Lineweaver-Burk plot. KM Vmax (S), uM 0.08 0.12 0.54 1.23 1.82 2.72 Reaction Velocity (UM/min) 0.15 0.21 0.70 1.1 1.3 15 4.94 10.00 1.8
i need help with all question 5 please! thank you Question 5: Use the data below...
i need help with all question 5 please! thank you
Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...
Please do everything like you were answering a test. Don’t attempt if youre not going to do all p...
Please do everything like you were answering a test. Don’t attempt
if youre not going to do all parts. Do it ASAP and I will give you
a good rating. If not I will report you. Thank you so much for
being the best. Show work if necessary and be concise. There’s no
way for me to separate so do all parts.
do all parts please. I cant seperate it because it will be
refunded.
2. i) (10 points) The...
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]....
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
The next 3 questions are related. The results of an enzyme analysis are: KM=1.1x10-5 M; VMAX=47.6...
The next 3 questions are related. The results of an enzyme analysis are: KM=1.1x10-5 M; VMAX=47.6 μM/min. What is the Lineweaver Burk plot equation (recall: y=1/Keq and x=1/[S])?
Nobody has been able to solve the Km. The Vmax values are correct. I believe I...
Nobody has been able to solve the Km. The Vmax values are
correct. I believe I have my graph wrong. Can someone please help
me solve this.
Using the Lineweaver-Burk Equation 1/Vo Km/Vmax[S]1/Vmax) create a graph of both the Non-Inhibited data and Inhibited data below (on the same graph axes) and calculate the KM and VMax for each line. Vo (umol/L.min) Vo (Hmol/L-min) [Sol (umol/L) Non-Inhibited Data Inhibited Data .00e-06 13.9 7.60 .00e-06 18.0 9.90 ..10e- 05 10e-05 26.0 14.8...
9. The following data were obtained from an enzyme kinetics experiment. Graph the data using a...
9. The following data were obtained from an enzyme kinetics experiment. Graph the data using a Lineweaver-Burk plot and determine the values for Km and Vmax. [S] (UM) V(nmol/min) 0.20 0.26 1.43 1.67 2.08 3.33 0.33 1.00
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vma...
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vmax and the amount of enzyme present in this experiment. Use excel to obtain the lineweaver burk plot. Substrate concentration (MM) 1 Initial velocity (umol/min) 167 250 334 376 498 499 100 1,000
How can the Michaelis-Menten constant, be derived from this Lineweaver-Burk plot? Vmax O km = (-1)/(x-intercept)...
How can the Michaelis-Menten constant, be derived from this Lineweaver-Burk plot? Vmax O km = (-1)/(x-intercept) O km = (-1) * (x-intercept) O km = 1/(x-intercept) 0 Km = s;lope
(I need help with part C, Drawing the expected Michaelis-Menten plot; Do NOT draw the Lineweaver-Burk...
(I need help with part C, Drawing the expected Michaelis-Menten plot; Do NOT draw the Lineweaver-Burk plot. thanks!) 1. Michaelis-Menten kinetics- use the M-M equation to answer the following: a. An enzyme (5 µM) has a Vmax of 450 mM/min. What is kcat? b. When the substrate concentration is 50 mM, the initial velocity (V0) was measured to be 375 mM/min. Under the conditions described above, calculate the KM. c. Draw the expected Michaelis-Menten plot (label your axes and include...
Name Page Number Date Vmax 1. Estimate Vmax and Km using the velocity vs. substrate concentration plot - KM 2. Calculate the Vmax and Km using the Lineweaver-Burk plot. KM Vmax (S), uM 0.08 0.12 0.54 1.23 1.82 2.72 Reaction Velocity (UM/min) 0.15 0.21 0.70 1.1 1.3 15 4.94 10.00 1.8
i need help with all question 5 please! thank you
Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...
Please do everything like you were answering a test. Don’t attempt
if youre not going to do all parts. Do it ASAP and I will give you
a good rating. If not I will report you. Thank you so much for
being the best. Show work if necessary and be concise. There’s no
way for me to separate so do all parts.
do all parts please. I cant seperate it because it will be
refunded.
2. i) (10 points) The...
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
Nobody has been able to solve the Km. The Vmax values are
correct. I believe I have my graph wrong. Can someone please help
me solve this.
Using the Lineweaver-Burk Equation 1/Vo Km/Vmax[S]1/Vmax) create a graph of both the Non-Inhibited data and Inhibited data below (on the same graph axes) and calculate the KM and VMax for each line. Vo (umol/L.min) Vo (Hmol/L-min) [Sol (umol/L) Non-Inhibited Data Inhibited Data .00e-06 13.9 7.60 .00e-06 18.0 9.90 ..10e- 05 10e-05 26.0 14.8...
9. The following data were obtained from an enzyme kinetics experiment. Graph the data using a Lineweaver-Burk plot and determine the values for Km and Vmax. [S] (UM) V(nmol/min) 0.20 0.26 1.43 1.67 2.08 3.33 0.33 1.00
1. The turnover number for an enzyme is known to be 5000 min. From the following set of data, calculate the Km, Vmax and the amount of enzyme present in this experiment. Use excel to obtain the lineweaver burk plot. Substrate concentration (MM) 1 Initial velocity (umol/min) 167 250 334 376 498 499 100 1,000
How can the Michaelis-Menten constant, be derived from this Lineweaver-Burk plot? Vmax O km = (-1)/(x-intercept) O km = (-1) * (x-intercept) O km = 1/(x-intercept) 0 Km = s;lope
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