![Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel.](http://img.homeworklib.com/questions/ec6ef8b0-6eee-11ea-95b4-9df4bad5752d.png?x-oss-process=image/resize,w_560)
Homework Answers
For parts a-c follow the following equations and concept.
According to Michaelis-Menten equation:-
![Vo=lVmax * [Km] + [S]](http://img.homeworklib.com/questions/ed0856d0-6eee-11ea-8457-7f3bd249d8ce.png?x-oss-process=image/resize,w_560)
Now, you may plot a curve in excel by putting Vo at y-axis and [S] at x-axis with the maximum value as Vmax. More are the values of Vo and [S] easier it is to find Vmax
If [S]=Km, then the initial velocity Vo= (Vmax)/2
For parts d-l follow the following equation (Line Weaver Berk Plot):-
![1 Vo Km 1 [Vmax] * [91 [V max](http://img.homeworklib.com/questions/ed61cd20-6eee-11ea-8ed0-f1208c9a3537.png?x-oss-process=image/resize,w_560)
It is plot as 1/Vo as y-axis and [S] at x-axis. (y=mx+c)
slope= Km/Vmax, intercept= 1/Vmax
Now plot your graph with the values provided in the table and calculate slope and thus, Km
Using this equation try to solve the numerical yourself. If you still find difficulty, do tell me!
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i need help with all question 5 please! thank you
Question 5: Use the data below...
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]....
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as a function of substrate concentration; label both axes. Write the associated Michaelis-Menton equation and show the location of Km and Vmax on your graph. b. Draw a second graph showing the classic Lineweaver-Burk plot; label both axes. Show the location of Km and Vmax on your graph. Discuss which plot is the most useful to determine Vmax. Draw a second line on each graph...
Where do the numbers come from for a Michaelis-Menton and Lineweaver-Burke Plot? Can you please explain...
Where do the numbers come from for a Michaelis-Menton and
Lineweaver-Burke Plot? Can you please explain in detail please and
thank you?
Did
an Enzyme lab w/dilutions and recorded absorbances. I am now asked
to construct Michaelis-Menton ans Lineweaver-Burke plots BUT I dont
know if the plot is based on my results from the dilutions and
absorbances.
For your lab reports, you will determine how much sugar was made during your enzyme reactions in Week 2 based on the linear...
(I need help with part C, Drawing the expected Michaelis-Menten plot; Do NOT draw the Lineweaver-Burk...
(I need help with part C, Drawing the expected Michaelis-Menten plot; Do NOT draw the Lineweaver-Burk plot. thanks!) 1. Michaelis-Menten kinetics- use the M-M equation to answer the following: a. An enzyme (5 µM) has a Vmax of 450 mM/min. What is kcat? b. When the substrate concentration is 50 mM, the initial velocity (V0) was measured to be 375 mM/min. Under the conditions described above, calculate the KM. c. Draw the expected Michaelis-Menten plot (label your axes and include...
The equation that describes the above Michaelis-Menten curve: Vo TS]+K Vmax [S] Michaelis-Menten Equation Lineweaver and...
The equation that describes the above Michaelis-Menten curve: Vo TS]+K Vmax [S] Michaelis-Menten Equation Lineweaver and Burke manipulated the Michaelis-Menten equation to yield: Ko V I S Vmax [S] Lineweaver-Burke Equation Linewenver Burke Equation If you plot 1/ V. vs. 1/[S], you get the following Lineweaver-Burke plot: 1/V. Slope = km/Vmax Intercept = -1/KM -Intercept = 1/Vmax 1/[S] Which is easier to calculate values for Km and Vmax, using the linear (y=mx+b) Lineweaver-Burke Plot or the Michaelis-Menten curve?
Please do everything like you were answering a test. Don’t attempt if youre not going to do all p...
Please do everything like you were answering a test. Don’t attempt
if youre not going to do all parts. Do it ASAP and I will give you
a good rating. If not I will report you. Thank you so much for
being the best. Show work if necessary and be concise. There’s no
way for me to separate so do all parts.
do all parts please. I cant seperate it because it will be
refunded.
2. i) (10 points) The...
4. The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is give...
4. The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by: 1 Km 1 1 where, the plot of (1/V.) vs (1/[S]) is a linear plot. If you only know the x-axis and y-axis intercepts from this plot, how can you determine Vmax and Km? (A) multiply the reciprocal of the x-axis intercept by -1. (B) multiply the reciprocal of the y-axis intercept by -1. (C) take the reciprocal of the x-axis intercept. (D) take...
A different experiment yields the following kinetic data Substrate] (mM)Vo (uM/min) no inhibitorVo (uM/min)+7 nM inhibitor...
A different experiment yields the following kinetic data Substrate] (mM)Vo (uM/min) no inhibitorVo (uM/min)+7 nM inhibitor 0.02 0.04 0.10 0.25 1.00 2.50 0.8 2.9 8.6 24 36 50 Plot the data for the kinetics of the enzyme (with and without the inhibitor) in a double reciprocal (Lineweaver-Burk) plot. Keep in mind that the x axis is 1/[S] and the y axis is 1/Vo. If you are using Excel you want to choose the (x,y) scatter plot (without a ne). You...
112 marks] 3. The relationship between initial velocity (V.) and substrate concentration of most of the...
112 marks] 3. The relationship between initial velocity (V.) and substrate concentration of most of the enzyme- catalized reactions are explained by Michaelis-Menten equation. IMPORTANT: Show the calculations and indicate the units for all your answers. a. For an enzyme which follows the Michaelis-Menten enzyme kinetics, Km is 50 mmol L. Calculate the substrate concentration required to obtain the initial velocity (V.) equivalent to 90% of the maximum velocity (Vmax). b. The Vmax of the above reaction is 250 mmol...
Michaelis-Menten plot and Lineweaver-Burk plot calculations: Use provided data to generate both M-M and L-B plots....
Michaelis-Menten plot and Lineweaver-Burk plot calculations: Use provided data to generate both M-M and L-B plots. Use scatter plots with markers on Excel: On the M-M Plot: estimate Vmax, KM On the L-B Plot: determine Vmax, KM, keat, kcat/Km. The total enzyme concentration is 5 uM. Graphs can be 1/2 page. Must be computer generated with all axes labeled. Substrate (mM) V. (mM/s) | 1/[S] (mM1) 1/V. (s/mM) 10 | 0 2.73 5.45 8.17 10.9 40.4 0.124 0.181 0.212 0.228...
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as a function of substrate concentration; label both axes. Write the associated Michaelis-Menton equation and show the location of Km and Vmax on your graph. b. Draw a second graph showing the classic Lineweaver-Burk plot; label both axes. Show the location of Km and Vmax on your graph. Discuss which plot is the most useful to determine Vmax. Draw a second line on each graph...
Where do the numbers come from for a Michaelis-Menton and
Lineweaver-Burke Plot? Can you please explain in detail please and
thank you?
Did
an Enzyme lab w/dilutions and recorded absorbances. I am now asked
to construct Michaelis-Menton ans Lineweaver-Burke plots BUT I dont
know if the plot is based on my results from the dilutions and
absorbances.
For your lab reports, you will determine how much sugar was made during your enzyme reactions in Week 2 based on the linear...
The equation that describes the above Michaelis-Menten curve: Vo TS]+K Vmax [S] Michaelis-Menten Equation Lineweaver and Burke manipulated the Michaelis-Menten equation to yield: Ko V I S Vmax [S] Lineweaver-Burke Equation Linewenver Burke Equation If you plot 1/ V. vs. 1/[S], you get the following Lineweaver-Burke plot: 1/V. Slope = km/Vmax Intercept = -1/KM -Intercept = 1/Vmax 1/[S] Which is easier to calculate values for Km and Vmax, using the linear (y=mx+b) Lineweaver-Burke Plot or the Michaelis-Menten curve?
Please do everything like you were answering a test. Don’t attempt
if youre not going to do all parts. Do it ASAP and I will give you
a good rating. If not I will report you. Thank you so much for
being the best. Show work if necessary and be concise. There’s no
way for me to separate so do all parts.
do all parts please. I cant seperate it because it will be
refunded.
2. i) (10 points) The...
4. The double-reciprocal transformation of the Michaelis-Menten equation, also called the Lineweaver-Burk plot, is given by: 1 Km 1 1 where, the plot of (1/V.) vs (1/[S]) is a linear plot. If you only know the x-axis and y-axis intercepts from this plot, how can you determine Vmax and Km? (A) multiply the reciprocal of the x-axis intercept by -1. (B) multiply the reciprocal of the y-axis intercept by -1. (C) take the reciprocal of the x-axis intercept. (D) take...
A different experiment yields the following kinetic data Substrate] (mM)Vo (uM/min) no inhibitorVo (uM/min)+7 nM inhibitor 0.02 0.04 0.10 0.25 1.00 2.50 0.8 2.9 8.6 24 36 50 Plot the data for the kinetics of the enzyme (with and without the inhibitor) in a double reciprocal (Lineweaver-Burk) plot. Keep in mind that the x axis is 1/[S] and the y axis is 1/Vo. If you are using Excel you want to choose the (x,y) scatter plot (without a ne). You...
112 marks] 3. The relationship between initial velocity (V.) and substrate concentration of most of the enzyme- catalized reactions are explained by Michaelis-Menten equation. IMPORTANT: Show the calculations and indicate the units for all your answers. a. For an enzyme which follows the Michaelis-Menten enzyme kinetics, Km is 50 mmol L. Calculate the substrate concentration required to obtain the initial velocity (V.) equivalent to 90% of the maximum velocity (Vmax). b. The Vmax of the above reaction is 250 mmol...
Michaelis-Menten plot and Lineweaver-Burk plot calculations: Use provided data to generate both M-M and L-B plots. Use scatter plots with markers on Excel: On the M-M Plot: estimate Vmax, KM On the L-B Plot: determine Vmax, KM, keat, kcat/Km. The total enzyme concentration is 5 uM. Graphs can be 1/2 page. Must be computer generated with all axes labeled. Substrate (mM) V. (mM/s) | 1/[S] (mM1) 1/V. (s/mM) 10 | 0 2.73 5.45 8.17 10.9 40.4 0.124 0.181 0.212 0.228...
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