Question

Where do the numbers come from for a Michaelis-Menton and Lineweaver-Burke Plot? Can you please explain in detail please and thank you?

Did an Enzyme lab w/dilutions and recorded absorbances. I am now asked to construct Michaelis-Menton ans Lineweaver-Burke plots BUT I dont know if the plot is based on my results from the dilutions and absorbances.

For your lab reports, you will determine how much sugar was made during your enzyme reactions in Week 2 based on the linear regression line determined from your standard curve from Week 2. Construct Michaelis-Menten and Lineweaver-Burke plots. Your observed absorbance is after 5 minutes of reaction time, therefore your initial rate (vo - Absorbance units/min) can be calculated as Observed absorbance/5. From your data, estimate Vmax and Km values.

Answer 1

Both plots are used in enzyme kinetics

A Michaelis-Menton Plot looks like this:

Vmax Reaction velocity v Km = Vmax/2 Substrate concentration (S]

And it is the representation of the equation:

$v=\frac{Vmax[S]}{Km + [S]}$

were:

v = reaction rate = the rate at which your product (the product of the enzymatic reaction) is forming

[S] = the concentration of the substrate

Vmax = the maximum reaction rate = this rate is equal to the saturation of the system were all the available enzyme is pair whit a substrate. And therefore can't go any higher.

Km = Michaelis - Mente constant = Vmax/2

So on your data you could find a point were the v/product formation does not go any higher, that would be your Vmax and you could know your Km

A Lineweaver-Burke Plot is a different take on the Michaelis-Menton Plot in which we change the equation so your v plots in a straight line;

$\frac{1}{v}=\frac{Km}{Vmax[S]} + \frac{1}{Vmax}$

were:

v = reaction rate = the rate at which your product (the product of the enzymatic reaction) is forming

[S] = the concentration of the substrate

Vmax = the maximum reaction rate = this rate is equal to the saturation of the system were all the available enzyme is pair whit a substrate. And therefore can't go any higher.

Km = Michaelis - Mente constant = Vmax/2

And the plot look like:

1/ Km/Vmax 1/Vmax 1/[S] -1/Km

Whit your data you should be able to find Vmax and there fore find Km