What would a Michaelis Menton Plot and a Lineweaver-Burke plot look like using this data?
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![Linweaver plot 0.15 0.14 0.13 0.12 1/o 0.04 0.02 0.01 0.00 0.010 0.013 0.020 0.040 1/[5]](http://img.homeworklib.com/questions/c0f370f0-ef57-11eb-b74f-150aeed16ab5.png?x-oss-process=image/resize,w_560)
Table 1.0: Enzyme Concentration Trial 1 (sec) Trial 2 (sec) Rate(1/Sec) Enzyme Concentration (Units/mL) 100 units/ml 10.30 sec 9.85 sec 10.075 75 units/mL 9.14 sec 8.70 sec 8.92 50 units/mL 8.78 sec 7.72 sec 8.25 25 units/mL 7.50 sec 7.36 sec 7.43
Michaelis menten graph Vo(1/Sec) 50 [S](Unit/mL)
Table 1.0: Enzyme Concentration Trial 1 (sec) Trial 2 (sec) Rate(1/Sec) Enzyme Concentration (Units/mL) 100 units/ml 10.30 sec 9.85 sec 10.075 75 units/mL 9.14 sec 8.70 sec 8.92 50 units/mL 8.78 sec 7.72 sec 8.25 25 units/mL 7.50 sec 7.36 sec 7.43
Michaelis menten graph 20.07 Vo(1/Sec) 100 [5](Unit/mL)
Linweaver plot 0.15 0.14 0.13 0.12 1/o 0.04 0.02 0.01 0.00 0.010 0.013 0.020 0.040 1/[5]
Table 1.0: Enzyme Concentration Trial 1 (sec) Trial 2 (sec) Rate(1/Sec) Enzyme Concentration (Units/mL) 100 units/ml 10.30 sec 9.85 sec 10.075 75 units/mL 9.14 sec 8.70 sec 8.92 50 units/mL 8.78 sec 7.72 sec 8.25 25 units/mL 7.50 sec 7.36 sec 7.43
Michaelis menten graph 20.07 Vo(1/Sec) 100 [5](Unit/mL)
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What would a Michaelis Menton Plot and a Lineweaver-Burke plot
look like using this data?
Use...
Where do the numbers come from for a Michaelis-Menton and Lineweaver-Burke Plot? Can you please explain...
Where do the numbers come from for a Michaelis-Menton and
Lineweaver-Burke Plot? Can you please explain in detail please and
thank you?
Did
an Enzyme lab w/dilutions and recorded absorbances. I am now asked
to construct Michaelis-Menton ans Lineweaver-Burke plots BUT I dont
know if the plot is based on my results from the dilutions and
absorbances.
For your lab reports, you will determine how much sugar was made during your enzyme reactions in Week 2 based on the linear...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as a function of substrate concentration; label both axes. Write the associated Michaelis-Menton equation and show the location of Km and Vmax on your graph. b. Draw a second graph showing the classic Lineweaver-Burk plot; label both axes. Show the location of Km and Vmax on your graph. Discuss which plot is the most useful to determine Vmax. Draw a second line on each graph...
The following observations come from Lineweaver-Burke plots, based on kinetic data generated from a Michaelis/Menton-type enzyme...
The following observations come from Lineweaver-Burke plots, based on kinetic data generated from a Michaelis/Menton-type enzyme (E) that catalyzes the hydrolysis of a peptide substrate (S). All data were generated in the presence of 18.0 μM total enzyme. The enzyme-catalyzed reaction has a Km of 3.00 μM and a Vmax of 2.00 μM/sec. The enzyme-catalyzed reaction in the presence of 15.0 μM of Inhibitor A has an apparent Km of 2.25 μM and an apparent Vmax of 1.50 μM/sec. The...
3.) Enzyme concentration is 3nmol/mL, a Lineweaver-Burke plot of the data gives a line of y...
3.) Enzyme concentration is 3nmol/mL, a Lineweaver-Burke plot of the data gives a line of y = 0.52870x + 0.00426 where the y intercept has units of umol' mL s. [S] uM vo (umol ml-15-1) 169 320 160 132 80.0 92.0 40.0 57.2 20.0 32.6 10.0 17.5 Calculate kcat for the reaction. Calculate Km for the enzyme.
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]....
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
thank you a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL...
thank you
a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL Velocity umole/(min-mL) 10 15 20 31 50 75 100 125 250 330 380 437 524 612 662 678 696 713 Have the substrate concentration as the x-axis of the graph, and the velocity as the y-axis. . Use a grid line every 10 substrate concentration units, and a grid line every 20 velocity units. . In Excel, gridlines can be controlled under the Chart...
Michaelis-Menten plot and Lineweaver-Burk plot calculations- use provided data to generate both M-M and L-B plots....
Michaelis-Menten plot and Lineweaver-Burk plot calculations- use provided data to generate both M-M and L-B plots. Use scatter plots with markers on Excel to determine Vinas, KM, kcat, kcat/KM. The total enzyme concentration is 5 μM. Graphs can be 2 page. Must be computer generated with all axes labeled. Substrate (mM Vo (mM/s) 2.73 5.45 8.17 10.9 40.4 0.124 0.181 0.212 0.228 0.303
Michaelis-Menten plot and Lineweaver-Burk plot calculations: Use provided data to generate both M-M and L-B plots....
Michaelis-Menten plot and Lineweaver-Burk plot calculations: Use provided data to generate both M-M and L-B plots. Use scatter plots with markers on Excel: On the M-M Plot: estimate Vmax, KM On the L-B Plot: determine Vmax, KM, keat, kcat/Km. The total enzyme concentration is 5 uM. Graphs can be 1/2 page. Must be computer generated with all axes labeled. Substrate (mM) V. (mM/s) | 1/[S] (mM1) 1/V. (s/mM) 10 | 0 2.73 5.45 8.17 10.9 40.4 0.124 0.181 0.212 0.228...
1- Describe two attributes that are different for hemoglobin and myoglobin; focus on characteristics that are...
1- Describe two attributes that are different for hemoglobin and myoglobin; focus on characteristics that are a direct consequence of differences in tertiary and/or quaternary structure. For each attribute, be sure to specifically explain how 3° and 4° organization impacts the described differences. 2- The kinetics of an enzyme (E)-catalyzed reaction are measured over a range of substrate (S) concentrations in the presence or absence of a reversible inhibitor (I). The total enzyme concentration is 10 uM, and the inhibitor...
1. Graphic depictions of enzyme kinetic data can aid in evaluation of reversible enzyme inhibition. Please...
1. Graphic depictions of enzyme kinetic data can aid in evaluation of reversible enzyme inhibition. Please use the data below to determine type of inhibition for using two different concentrations (in blue and purple). Be sure to include graphical representation (Michaelis-Menton, Lineweaver-Burk plots), type of inhibition, appropriate kinetic schemes and rate equations. The enzyme concentration at its initial state E, was kept constant. All data points are given in arbitrary units. Completely justify your answer. Use Excel or graphing paper...
Where do the numbers come from for a Michaelis-Menton and
Lineweaver-Burke Plot? Can you please explain in detail please and
thank you?
Did
an Enzyme lab w/dilutions and recorded absorbances. I am now asked
to construct Michaelis-Menton ans Lineweaver-Burke plots BUT I dont
know if the plot is based on my results from the dilutions and
absorbances.
For your lab reports, you will determine how much sugar was made during your enzyme reactions in Week 2 based on the linear...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as a function of substrate concentration; label both axes. Write the associated Michaelis-Menton equation and show the location of Km and Vmax on your graph. b. Draw a second graph showing the classic Lineweaver-Burk plot; label both axes. Show the location of Km and Vmax on your graph. Discuss which plot is the most useful to determine Vmax. Draw a second line on each graph...
3.) Enzyme concentration is 3nmol/mL, a Lineweaver-Burke plot of the data gives a line of y = 0.52870x + 0.00426 where the y intercept has units of umol' mL s. [S] uM vo (umol ml-15-1) 169 320 160 132 80.0 92.0 40.0 57.2 20.0 32.6 10.0 17.5 Calculate kcat for the reaction. Calculate Km for the enzyme.
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
thank you
a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL Velocity umole/(min-mL) 10 15 20 31 50 75 100 125 250 330 380 437 524 612 662 678 696 713 Have the substrate concentration as the x-axis of the graph, and the velocity as the y-axis. . Use a grid line every 10 substrate concentration units, and a grid line every 20 velocity units. . In Excel, gridlines can be controlled under the Chart...
Michaelis-Menten plot and Lineweaver-Burk plot calculations- use provided data to generate both M-M and L-B plots. Use scatter plots with markers on Excel to determine Vinas, KM, kcat, kcat/KM. The total enzyme concentration is 5 μM. Graphs can be 2 page. Must be computer generated with all axes labeled. Substrate (mM Vo (mM/s) 2.73 5.45 8.17 10.9 40.4 0.124 0.181 0.212 0.228 0.303
Michaelis-Menten plot and Lineweaver-Burk plot calculations: Use provided data to generate both M-M and L-B plots. Use scatter plots with markers on Excel: On the M-M Plot: estimate Vmax, KM On the L-B Plot: determine Vmax, KM, keat, kcat/Km. The total enzyme concentration is 5 uM. Graphs can be 1/2 page. Must be computer generated with all axes labeled. Substrate (mM) V. (mM/s) | 1/[S] (mM1) 1/V. (s/mM) 10 | 0 2.73 5.45 8.17 10.9 40.4 0.124 0.181 0.212 0.228...
1. Graphic depictions of enzyme kinetic data can aid in evaluation of reversible enzyme inhibition. Please use the data below to determine type of inhibition for using two different concentrations (in blue and purple). Be sure to include graphical representation (Michaelis-Menton, Lineweaver-Burk plots), type of inhibition, appropriate kinetic schemes and rate equations. The enzyme concentration at its initial state E, was kept constant. All data points are given in arbitrary units. Completely justify your answer. Use Excel or graphing paper...
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