Homework Answers
![Lineweaver-Burk Equation 1 1 + ro kcat [E],kcat [El,[S]. Thus the plot of and sto wll yield a straight line with slope and y](http://img.homeworklib.com/questions/d3c61300-e8c8-11ea-8f3a-99fefb9c5382.png?x-oss-process=image/resize,w_560)
![1 Lineweaver-Burk Equation 1 + ro kcat [E]okcat [E],[S]. Thus the plot of 1 and 57. wll yield a straight line with slope kca](http://img.homeworklib.com/questions/d4212890-e8c8-11ea-85bc-773f95924b2b.png?x-oss-process=image/resize,w_560)
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3.) Enzyme concentration is 3nmol/mL, a Lineweaver-Burke plot of the data gives a line of y...
(b.) calculate KM for the enzyme (c) When the reactions in part (b) are repeated in...
(b.) calculate KM for the enzyme
(c) When the reactions in part (b) are repeated in the presence
of 12 microm of an uncompetitive inhibitor, the y-intercept of the
Lineweaver-Burk plot is 0.352 (micromol-1 ml s). Calculate K'I for
this inhibitor.
[S] UM 320 160 80.0 40.0 20.0 10.0 Volumol nk-1 S-1) 169 132 92.0 57.2 32.6 17.5
The Lineweaver-Burke Plot for the enzyme in question 1 is shown below. In this plot, the...
The Lineweaver-Burke Plot for the enzyme in question 1 is shown below. In this plot, the y intercept (0.019153) is 1/vmax and the x intercept (-0.0449) is -1/km. What are the exact km and vmax based on this graph (show your work)? Km= Vmax=
how do you make a lineweaver-burk plot where the trend line extends backwards? is there a...
how do you make a lineweaver-burk plot where the trend line
extends backwards? is there a way of figuring out how far back it
should extend based of your data ? As well, how do you plot two
sets of data on one graph ? Thank you! (idk if you need to see my
data-> attached below)
Biochemistry Enzyme Kinetics Assignment Four answers for this assignment will be completed in elearn: En in elearn: Enzyme Kinetics Quiz ots but must...
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]....
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
3. Use the below tables to complete lab calculations on the worksheet on pages 2-6 of...
3. Use the below tables to complete lab calculations on the worksheet on pages 2-6 of this document. You will submit the worksheet via dropbox on Canvas by you assigned lab time the week of March 30th through April 3rd. A document with sample calculations of different concentrations is provided to you on canvas. Enzyme Kinetics Lab Buffer volume (mL) Enzyme Volume (ml) Substrate Volume (ml) TOTAL VOLUME (mL) 0.04 0.5 1.5 0.04 0.25 1.5 0.04 0.1 1.5 0.04 0.05...
(b.) calculate KM for the enzyme
(c) When the reactions in part (b) are repeated in the presence
of 12 microm of an uncompetitive inhibitor, the y-intercept of the
Lineweaver-Burk plot is 0.352 (micromol-1 ml s). Calculate K'I for
this inhibitor.
[S] UM 320 160 80.0 40.0 20.0 10.0 Volumol nk-1 S-1) 169 132 92.0 57.2 32.6 17.5
how do you make a lineweaver-burk plot where the trend line
extends backwards? is there a way of figuring out how far back it
should extend based of your data ? As well, how do you plot two
sets of data on one graph ? Thank you! (idk if you need to see my
data-> attached below)
Biochemistry Enzyme Kinetics Assignment Four answers for this assignment will be completed in elearn: En in elearn: Enzyme Kinetics Quiz ots but must...
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
3. Use the below tables to complete lab calculations on the worksheet on pages 2-6 of this document. You will submit the worksheet via dropbox on Canvas by you assigned lab time the week of March 30th through April 3rd. A document with sample calculations of different concentrations is provided to you on canvas. Enzyme Kinetics Lab Buffer volume (mL) Enzyme Volume (ml) Substrate Volume (ml) TOTAL VOLUME (mL) 0.04 0.5 1.5 0.04 0.25 1.5 0.04 0.1 1.5 0.04 0.05...
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