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a) Do a Michaelis-Menton plot of the enzyme assay data below. Substrate Concentration mg/mL...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as a function of substrate concentration; label both axes. Write the associated Michaelis-Menton equation and show the location of Km and Vmax on your graph. b. Draw a second graph showing the classic Lineweaver-Burk plot; label both axes. Show the location of Km and Vmax on your graph. Discuss which plot is the most useful to determine Vmax. Draw a second line on each graph...
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]....
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
i need help with all question 5 please! thank you Question 5: Use the data below...
i need help with all question 5 please! thank you
Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...
please and thank you! Volume of K2Cr04 (mL) Absorbance Molar concentration of CrO42- (M) 2.4 x...
please and thank you!
Volume of K2Cr04 (mL) Absorbance Molar concentration of CrO42- (M) 2.4 x 10-5 1.00 0.89 5.00 1.2*10^-4 0.55 10.00 2.4*10^-4 0.99 15.00 3.6*10^-4 1.37 QUESTION 3 10 points Save Answer Upload the plot of the standard curve. Use excel to construct it. Label the axes and draw the linear relationship. Show the equation of the line on the plot (at the top right away from the data points). Do not label the data points Hint: Once...
how do you make a lineweaver-burk plot where the trend line extends backwards? is there a...
how do you make a lineweaver-burk plot where the trend line
extends backwards? is there a way of figuring out how far back it
should extend based of your data ? As well, how do you plot two
sets of data on one graph ? Thank you! (idk if you need to see my
data-> attached below)
Biochemistry Enzyme Kinetics Assignment Four answers for this assignment will be completed in elearn: En in elearn: Enzyme Kinetics Quiz ots but must...
Do case #4 Background: The data shown on the following page was obtained from Tension Tests...
Do
case #4
Background: The data shown on the following page was obtained from Tension Tests of 10 unknown ductile metals. For each test, various dimensions and loads were measured before and after failure. The data is a summary of readings taken during the test (loads vs extensometer readings). Purpose: To determine the stress, strain, yield stress and modulus of elasticity of a circular shaped specimen from a tension test. Directions: A. For your particular case (depending on your last...
I need help to write a nice introduction for experiment 6 please ( no hands write ) typing Thank you HEAT TREATMENT OF STEELS EXPERIMENT 6 EXPERIMENT 6 HEAT TREATMENT OF STEELS THEORY The Ef...
I need help to write a nice introduction for experiment 6
please ( no hands write ) typing
Thank you
HEAT TREATMENT OF STEELS EXPERIMENT 6 EXPERIMENT 6 HEAT TREATMENT OF STEELS THEORY The Effect of Cooling Rate One of the most convenient methods for controlling the properties of a given steel, i.e., a steel whose composition is already fixed, consists of austenizing the steel and ten cooling to room temperature at some predetermined rate. A variation of cooling rates...
1. MICHAELIS-MENTON-(REQUIRED) a. Draw a simple graph, showing the classic Michaelis-Menton plot of enzyme activity as a function of substrate concentration; label both axes. Write the associated Michaelis-Menton equation and show the location of Km and Vmax on your graph. b. Draw a second graph showing the classic Lineweaver-Burk plot; label both axes. Show the location of Km and Vmax on your graph. Discuss which plot is the most useful to determine Vmax. Draw a second line on each graph...
ISU Question 3: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo 1/[S1 1/V0 UM (UM/s) M (s/uM) 340 10 2.94E-03 0.2 530 740 0.8 910 1.6 1040 0.4 a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest (SD? c) Using your Vmax estimate, calculate 14 Vmax, and using your curve, estimate Km....
i need help with all question 5 please! thank you
Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...
please and thank you!
Volume of K2Cr04 (mL) Absorbance Molar concentration of CrO42- (M) 2.4 x 10-5 1.00 0.89 5.00 1.2*10^-4 0.55 10.00 2.4*10^-4 0.99 15.00 3.6*10^-4 1.37 QUESTION 3 10 points Save Answer Upload the plot of the standard curve. Use excel to construct it. Label the axes and draw the linear relationship. Show the equation of the line on the plot (at the top right away from the data points). Do not label the data points Hint: Once...
how do you make a lineweaver-burk plot where the trend line
extends backwards? is there a way of figuring out how far back it
should extend based of your data ? As well, how do you plot two
sets of data on one graph ? Thank you! (idk if you need to see my
data-> attached below)
Biochemistry Enzyme Kinetics Assignment Four answers for this assignment will be completed in elearn: En in elearn: Enzyme Kinetics Quiz ots but must...
Do
case #4
Background: The data shown on the following page was obtained from Tension Tests of 10 unknown ductile metals. For each test, various dimensions and loads were measured before and after failure. The data is a summary of readings taken during the test (loads vs extensometer readings). Purpose: To determine the stress, strain, yield stress and modulus of elasticity of a circular shaped specimen from a tension test. Directions: A. For your particular case (depending on your last...
I need help to write a nice introduction for experiment 6
please ( no hands write ) typing
Thank you
HEAT TREATMENT OF STEELS EXPERIMENT 6 EXPERIMENT 6 HEAT TREATMENT OF STEELS THEORY The Effect of Cooling Rate One of the most convenient methods for controlling the properties of a given steel, i.e., a steel whose composition is already fixed, consists of austenizing the steel and ten cooling to room temperature at some predetermined rate. A variation of cooling rates...
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