Question

112 marks] 3. The relationship between initial velocity (V.) and substrate concentration of most of the enzyme- catalized reactions are explained by Michaelis-Menten equation. IMPORTANT: Show the calculations and indicate the units for all your answers. a. For an enzyme which follows the Michaelis-Menten enzyme kinetics, Km is 50 mmol L. Calculate the substrate concentration required to obtain the initial velocity (V.) equivalent to 90% of the maximum velocity (Vmax).

Answer 1

Michaelis Menten equation to max is 1 KM+S voz initial velocity km a Michaou's constant Vmax r maximum voluulty Isl- en ty me concentration E a. K: vo Vmax [] UMA [s] CS max - KM + (3] JAN Stop is , 1065) = 450 +913] on [s] = 450 mmol/L Vo Ts] b. um +(3] 15) 50+ (5) adly? 50 + 30 + 3) = 100 50 9031 50 ] TS] : 5.55 mmol 4-9 na C et to vo vnax (s] KM+] B] 21 mol/l = 1000 mmol/l Substnate concentration very high than the case y here from that a sove on we com Vmax will be less thom the pneving case previon Predien

(d) from newearer. Bunk equ for competitive inhibitor presence of q (pre se me on) inhibiton а SSе и се innibiton Yo da intrebiton So, rmax so called Vmax remain same as above il = 250 mmol/lit/min. Kní = km ( a + (27) um = um in puesence of inhibition So, K'N >UM @CA4 So (1), Ито So, um will increase.