Your Mueller-Hinton agar is 3.5 mm in depth. How will this effect the results? Will they...
Your Mueller-Hinton agar is 3.5 mm in depth. How will this effect the results? Will they be falsely sensitive or falsely resistant? Will the zones be too large or too small? Explain your answer.
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Your Mueller-Hinton agar is 3.5 mm in depth. How will
this effect the results? Will they...
What effect would varying the standardized depth of the Mueller-Hinton agar have on sensitivity testing?
What effect would varying the standardized depth of the Mueller-Hinton agar have on sensitivity testing?
1. Of the 4 labeled disinfectants, which have antimicrobial activity and which do not? 2. Of...
1. Of the 4 labeled disinfectants, which have antimicrobial activity and which do not? 2. Of those that have antimicrobial activity, which one has the greatest? 9. 3. What is present in the plate that indicates antimicrobial activity? 4. Could there be any reason other than "significant antimicrobial activity that a zone of inhibition is very large? shutterstock.com . 1461769682 SLIDE 3 KIRBY-BAUER ANTIBIOTIC SENSITIVITY PLATE USING MEULLER-HINTON AGAR 1. What does each disc have a precise amount of? 2....
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The...
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
Small colonies are observed at the inner edge of the zone of inhibition around the oxacillin...
Small colonies are observed at the inner edge of the zone of inhibition around the oxacillin disk when a S. aureus is tested. The outer zone size corresponds to an interpretation of susceptible andthe small colonies are growing in the resistant range. How will you interpret the results, S (sensitive), I (intermediate), or R (resistant)? Explain your answer.
1. Someone new is preparing media for lab and adds buffers to the EMB agar that...
1. Someone new is preparing media for lab and adds buffers to the EMB agar that raise the pH to 7.8. The starting pH of EMB plates should be 6.9 – 7.3. No one is aware of the change. The EMB plates are inoculated for identification of unknown bacteria. After the appropriate incubation time, the results are interpreted. A. Discuss the consequences of the change in starting pH to the interpretation of the results. (The answer is not that no...
Consider these possible sources of error described below. Briefly explain the effect of each on your...
Consider these possible sources of error described below. Briefly explain the effect of each on your calculated molar heats of neutralization. Indicate which item(s) of data would be in error and the effect (too large, too small or no effect). a. Step 9: You failed to adjust the temperature of the acid and base to the same temperature before you mixed them in Step 10.
Describe and explain the possible effect on your results if the following experimental errors or variations...
Describe and explain the possible effect on your results if the following experimental errors or variations occurred. You used pure water as the reaction solvent. You started with 2.5mmol of vanillin and assumed that a 100% excess of NaBH_4 was 5.0 mmol and used that amount. Explain how to differentiate between the IR spectra of vanillin and vanillyl alcohol.
can you please help me with Qs 1-6 thanks ! LAB EXERCISE #6: Kirby-Bauer Disk Diffusion...
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
Consider the effect on your experimental results of the following and explain what the effect would...
Consider the
effect on your experimental results of the following and explain
what the effect would be and why.
a) The reaction of
BB- and OCl- turned out to be strongly
exothermic.
i don't really know
how this would change the absorbance levels over time. Is this
reaction already exotermic or is it endothermic.
results
We were unable to transcribe this imageD E F G H I J K L M N O P 1 Time (s) Absorbance In (Absorbance)...
catalase test EXPERIMENT 30 LAB REPORT OBSERVATIONS AND RESULTS Record your observations in the following chart....
catalase test
EXPERIMENT 30 LAB REPORT OBSERVATIONS AND RESULTS Record your observations in the following chart. Bacteria Foaming or No Foaming Catalase Present Absent M. luteus S. aureus S. lactis 1. Review Questions: 1. Explain why the enzyme catalase is essential for the survival of many aerobic and facultative anaerobic organisms? 2. Explain how catalase negative bacteria are capable of growing in the presence of oxygen Experiment 30 Catalase Test 273 3. Can bacterial cultures grown on blood agar be...
1. Of the 4 labeled disinfectants, which have antimicrobial activity and which do not? 2. Of those that have antimicrobial activity, which one has the greatest? 9. 3. What is present in the plate that indicates antimicrobial activity? 4. Could there be any reason other than "significant antimicrobial activity that a zone of inhibition is very large? shutterstock.com . 1461769682 SLIDE 3 KIRBY-BAUER ANTIBIOTIC SENSITIVITY PLATE USING MEULLER-HINTON AGAR 1. What does each disc have a precise amount of? 2....
A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of...
Consider these possible sources of error described below. Briefly explain the effect of each on your calculated molar heats of neutralization. Indicate which item(s) of data would be in error and the effect (too large, too small or no effect). a. Step 9: You failed to adjust the temperature of the acid and base to the same temperature before you mixed them in Step 10.
Describe and explain the possible effect on your results if the following experimental errors or variations occurred. You used pure water as the reaction solvent. You started with 2.5mmol of vanillin and assumed that a 100% excess of NaBH_4 was 5.0 mmol and used that amount. Explain how to differentiate between the IR spectra of vanillin and vanillyl alcohol.
can
you please help me with Qs 1-6
thanks !
LAB EXERCISE #6: Kirby-Bauer Disk Diffusion Test for Antibiotie Sensitivity Determination The Kirby Bauer Test is an agar diffusion test that is used to determine the effectiveness of antibiotics killing various species of bacteria. Filter paper disks saturated with the antibiotic of interest are placed a Mueller-Hinton agar plate on which bacteria that has been isolated from a clinical sample has been sarcad. The antibiotic then diffuses from the disk...
Consider the
effect on your experimental results of the following and explain
what the effect would be and why.
a) The reaction of
BB- and OCl- turned out to be strongly
exothermic.
i don't really know
how this would change the absorbance levels over time. Is this
reaction already exotermic or is it endothermic.
results
We were unable to transcribe this imageD E F G H I J K L M N O P 1 Time (s) Absorbance In (Absorbance)...
catalase test
EXPERIMENT 30 LAB REPORT OBSERVATIONS AND RESULTS Record your observations in the following chart. Bacteria Foaming or No Foaming Catalase Present Absent M. luteus S. aureus S. lactis 1. Review Questions: 1. Explain why the enzyme catalase is essential for the survival of many aerobic and facultative anaerobic organisms? 2. Explain how catalase negative bacteria are capable of growing in the presence of oxygen Experiment 30 Catalase Test 273 3. Can bacterial cultures grown on blood agar be...
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