Question

A clinical researcher performed a modified Kirby-Bauer assay on a bacterium cultured from a patient. The following is a summary of the assay performed: The clinician spread the surface of several Mueller-Hinton agar plates (a media type containing beef extract, casein, and starch) with the bacterial culture using aseptic technique. Next, they applied sterile antibiotic disks, each containing a different antibiotic to the surface of the plates. After the agar plates were incubated overnight, the clinician measured the diameter of cleared space with no bacterial growth around the antibiotic disk. The results are located below. Patient 453H Mechanism of action Is the patient sample restant? Diameter of clearing (mm) 9 24 Antibiotic Amoxicillin Ciprofloxacin Clindamycin Erythromycin Penicillin Vancomycin 23 26 16 17 1 The clinician was told the cultured bacterium was Staphylococcus aureus, a common skin microbe that can cause infection. 1. Using your textbook and/or the internet, determine the mechanism of action of each antibiotic. (The mechanism of action is how the antibiotic acts on the bacterial cell.) Fill in the mechanism in the above chart. 2. Using the Zone of Inhibition chart below (on the next page), determine if the patient sample is resistant to the antibiotic tested for. Fill your answer in the patient chart above. 3. Using the information from your completed patient chart, hypothesize if this infection would be susceptible to the use of the antibiotic Loracarbef. Why or why not?

Answer 1

Solution Hello, Today munbiology was we are going to discuss about based question. In here, 6 antibiotics, and its diameter of zone of inhibition that shown by Staphylocour uus aureus gwen cution each 1) in his question, the mechanism antibioties have to be write on the table. And I wrote it on the table given of of

by of Lone of of Lone of is sensitive organism is 2) and in this section, the resistance many of organism to each antibiotic have to be write. aand this is determined the diameter inlibition. the organism shows higher diameter inhibition, this this shows that to the antibiotu Because eit in low concentration antibiotie . of they show small diameter , then that shows that are resistant to the antibiotius. o And based on the chart whether the organism is resistant or not. even grow of they given I wrote

I Antibiotic Mechanism Action Diameter of clearing (mm) is the patient sample resistant! Yes 9 29 No Amoxicillin inhibits synthesis of bacterial cell wall . Ciprofloxacin finhibit DNA Synthesis by inhibiting PNA ggrase topásomerasen (clindamyin pisaute prekcin 23 to ribosomal subunit and No Synthesis 950s binding to

No synthesis b to sos 16 Yes Erythromycin Dissupts, protein 26 binding ribosomal subunit Penicillin let bind to trans peptidase which linhibits cell wall synthesis Nancomycin Inhibits the incorporation of N-acetyl muramic and and this results in the inhibition of cell wall Synthesis 11 No

an synthesis by pencullin of the patient say that 3) Lorauarbey is antibiotic which works like penicillin. This antibiotic inhibits cell wall inhibiting the binding proteins from the antibiotic resistance that given in the chart, we can the antibiotie Penicillin and Amoxicillin are not susuptible. These antibioties can't be used to treat The horrearbel is also function like Penuillin and Amoxicillin so So the horas is also acasbe net susceptible to the organism oso we can't use this antibiotic to treat this Bagamén Thank aureus. 0 m. you

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