Homework Answers
interpret the sputum gram stain
C. 2+-3gram+ diplococci
2+.3+WBCs
in this we find gram negative bacteria range in shape of thin rods and round shaped wbcs
gram negative diplococci is consider as normal flora. in this specimen with gram positive cocci, gram positive bacilli , gram negative coccobacilli.
these sputum specimen should be screened by gram stain for a routine culture.
morphotypes of normal flora should be lumping rather than individually
18. patient most likely infected with the normal flora
in this normal flora reported with the neutrophils, seen in inter celluraly.
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Sputum Gram Stain 17. Interpret the gram stain. A. 2+-3+ gram +rods 2+-3+ WBCs B. 2+-3+...
Sputum Gram Stain 17. Interpret the gram stain. A. 2+-3+ gram + rods 2+-3+ WBCs B....
Sputum Gram Stain 17. Interpret the gram stain. A. 2+-3+ gram + rods 2+-3+ WBCs B. 2+-3+ gram - rods 2+3+ epithelial cells C2+-3+ gram + diplococci 2+-3+ WBCS D. 2+-3+ gram+ cocci in clusters 1+ gram - rods 2+3+ WBCs 18. What is pt most likely infected with? A. pathogern Spring 2018 B./normal flora
Gram Stain of Pus from Genitalia 19. Interpret the gram stain. - A. 2+ gram+cocci, 2+...
Gram Stain of Pus from Genitalia 19. Interpret the gram stain. - A. 2+ gram+cocci, 2+ WBCs - B. 2+ gram- cocci, 2+ WBCs - C. 2+ gram- intracellular diplococci, 2+ WBCs - D. 2+ gram- rods, 2+ WBCs 20. Is this suggestive of normal flora or pathogenic flora? A. Normal flora because the body is not sterile B. Pathogenic flora because of presence of abnormally high WBCs Spring 2018
126. When you focus your Gram stain under 100x you see nice large purple rods with...
126. When you focus your Gram stain under 100x you see nice large purple rods with 80% pink ovals and other pink shapes. What is the most likely reason that a pure culture has more than one shape? (a) contamination because students that do not heat the entire loop (b) the microbe is Bacillus that is 52 hrs old (c) the slant is a 12 hr Clostridium (d) MYCOBACTERIA again! (e) none of these you "bumed" the smear in heat...
6) The Gram stain will stain bacteria on the basis of the chemistry of the: a)...
6) The Gram stain will stain bacteria on the basis of the chemistry of the: a) plasma membranes b) chromosomes c) cell wall d cytoplasmic proteins e) ribosomes 7) The Acid-Fast stain will stain bacteria on the basis of the chemistry of the a) plasma membranes b) chromosomes c) cell wall d) cytoplasmic proteins e) ribosomes 8) The morphology or shape of bacteria that are streptococcal or staphylococcal is dependant of the bacterial: a) cell wall b) plasma membrane c)...
Make 3 dichotomy keys using table: 1. Gram Negative Rods (5 Microbes) 2. Gram positive cocci...
Make 3 dichotomy keys using table:
1. Gram Negative Rods (5 Microbes)
2. Gram positive cocci
3. Gram positive rods (3 microbes)
DNASE test?
+ + + + + + + + + + + + 1 + PR Glu PR Lac MR VP Citrate Starch Gelatin E. coli AG AG P. hauseri A E. aerogenes A A K. pneumoniae AG AG A. faecalis E. faecalis A A K. rhizophila S. aureus A A S. epidermidis A A B. subtilis...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
Case Study #1 Clinical history: An HIV positive male presented in clinic with confusion and disorientation. He had a fev...
Case Study
#1
Clinical
history: An HIV positive male presented in clinic with
confusion and disorientation. He had a fever 38.5°C and
photophobia. His CD4 T cell count was 80/ul. An MRI and lumbar
puncture were performed. The MRI showed various small lesions
within the brain. CSF analysis indicated 32 White Blood Cells/ul
with 89% lymphocytes, and 6% monocytes, glucose of 22mg/dl, and
protein of 89mg/dl. Gram stain showed yeast and India ink negative
stain showed a thick capsule.
Image...
2. A middle-aged man presents to the emergency room with chest pain and is found to...
2. A middle-aged man presents to the emergency room with chest pain and is found to have suffered a heart attack. He has a past history of hypertension, non-insulin-dependent diabetes mellitus (NIDDM), and high cholesterol. Medical evaluation suggested the need for a triple bypass procedure. This is performed on the fifth day of his hospital stay. During recovery, he develops septic shock with fever and pneumonia. Gram-positive cocci in clusters, isolated from both his lungs (via sputum) and the surgical...
Case #1 Three individuals from a military unit involved in a large training exercise in a...
Case #1 Three individuals from a military unit involved in a large training exercise in a very remote area of the western US developed mild fevers, headaches and dry coughs. During subsequent days, the three individuals began to produce small amounts of thin, clear sputum. The small field hospital unit performed CBCs, sputum Gram stains, and chest x‐rays on the 3 patients. The CBCs were normal. The sputum gram stains showed a few neutrophils but no bacteria. The chest x‐rays...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
Sputum Gram Stain 17. Interpret the gram stain. A. 2+-3+ gram + rods 2+-3+ WBCs B. 2+-3+ gram - rods 2+3+ epithelial cells C2+-3+ gram + diplococci 2+-3+ WBCS D. 2+-3+ gram+ cocci in clusters 1+ gram - rods 2+3+ WBCs 18. What is pt most likely infected with? A. pathogern Spring 2018 B./normal flora
Gram Stain of Pus from Genitalia 19. Interpret the gram stain. - A. 2+ gram+cocci, 2+ WBCs - B. 2+ gram- cocci, 2+ WBCs - C. 2+ gram- intracellular diplococci, 2+ WBCs - D. 2+ gram- rods, 2+ WBCs 20. Is this suggestive of normal flora or pathogenic flora? A. Normal flora because the body is not sterile B. Pathogenic flora because of presence of abnormally high WBCs Spring 2018
126. When you focus your Gram stain under 100x you see nice large purple rods with 80% pink ovals and other pink shapes. What is the most likely reason that a pure culture has more than one shape? (a) contamination because students that do not heat the entire loop (b) the microbe is Bacillus that is 52 hrs old (c) the slant is a 12 hr Clostridium (d) MYCOBACTERIA again! (e) none of these you "bumed" the smear in heat...
6) The Gram stain will stain bacteria on the basis of the chemistry of the: a) plasma membranes b) chromosomes c) cell wall d cytoplasmic proteins e) ribosomes 7) The Acid-Fast stain will stain bacteria on the basis of the chemistry of the a) plasma membranes b) chromosomes c) cell wall d) cytoplasmic proteins e) ribosomes 8) The morphology or shape of bacteria that are streptococcal or staphylococcal is dependant of the bacterial: a) cell wall b) plasma membrane c)...
Make 3 dichotomy keys using table:
1. Gram Negative Rods (5 Microbes)
2. Gram positive cocci
3. Gram positive rods (3 microbes)
DNASE test?
+ + + + + + + + + + + + 1 + PR Glu PR Lac MR VP Citrate Starch Gelatin E. coli AG AG P. hauseri A E. aerogenes A A K. pneumoniae AG AG A. faecalis E. faecalis A A K. rhizophila S. aureus A A S. epidermidis A A B. subtilis...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
Case Study
#1
Clinical
history: An HIV positive male presented in clinic with
confusion and disorientation. He had a fever 38.5°C and
photophobia. His CD4 T cell count was 80/ul. An MRI and lumbar
puncture were performed. The MRI showed various small lesions
within the brain. CSF analysis indicated 32 White Blood Cells/ul
with 89% lymphocytes, and 6% monocytes, glucose of 22mg/dl, and
protein of 89mg/dl. Gram stain showed yeast and India ink negative
stain showed a thick capsule.
Image...
2. A middle-aged man presents to the emergency room with chest pain and is found to have suffered a heart attack. He has a past history of hypertension, non-insulin-dependent diabetes mellitus (NIDDM), and high cholesterol. Medical evaluation suggested the need for a triple bypass procedure. This is performed on the fifth day of his hospital stay. During recovery, he develops septic shock with fever and pneumonia. Gram-positive cocci in clusters, isolated from both his lungs (via sputum) and the surgical...
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