Question

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Q4. Why is the Kinyoun acid fast stain procedure referred to as the” acid fast stain procedure?

Answer 1

Ans) The Kinyoun method, or Kinyoun stain (cold method), developed by Robert Koch in 1882 and later modified by other scientist, is an acid-fast procedure used to stain any species of the genus Mycobacterium, Nocardia and Cryptosporidium species.

- Certain species of bacteria have a waxy lipid called mycolic acid, in their cell walls which allow them to be stained with Acid-Fast better then a Gram-Stain.

- The unique ability of mycobacteria to resist decolorization byacid-alcohol is why they are termed acid-fast.

- It involves the application of a primary stain (basic fuchsin), a decolorizer (acid-alcohol), and a counterstain (methylene blue).

- Unlike the Ziehl-Neelsen stain (Z-N stain), the Kinyoun method of staining does not require heating. In the Ziehl-Neelsen stain, heat acts as a physical mordant while phenol (carbol of carbol fuschin) acts as the chemical mordant.

- Since the Kinyoun stain is a cold method (no heat applied), the concentration of carbol fuschin used is increased.