Homework Answers
7. This image shows results of a Gram stain.
8. Crystal blue is the primary stain for gram-staining.
9. Given Organisms :
I. is Gram-positive (stained purple)
II. is cocci (spherical-shaped)
III. Have staph arrangement.
Therefore, from the above deductions we can say that the organism shown is " STAPHYLOCOCCUS".
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• 7. This image shows results of a(n). stain: A. Spore; B. Acid Fast; C. Gram;...
• 7. This image shows results of a(n) stain: A. Spore; B. Acid Fast; C. Gram;...
• 7. This image shows results of a(n) stain: A. Spore; B. Acid Fast; C. Gram; D. Capsule 8. is the primary stain for this procedure. 9. Identify the organisms shown as to staining reaction and morphology.
MICROBIOLOGY: Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential...
MICROBIOLOGY:
Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after...
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after you have viewed your acid fast stain slides. Bacteria for this exercise are: Mycobacterium smegmatis (or M. phlei) and S. aureus. Q1. Acid fast bacteria are have some significant differences as compared to non-acid fast bacteria. What are the major chemical (structural) differences among these different bacteria that might explain the acid-fast stain? Hint: This structural difference also affects the way the bacteria grow...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order,...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
Questions What does the term "Acid Fa" mean? Are acid-Fast organisms Gram-positive or Gram- 2 Do...
Questions What does the term "Acid Fa" mean? Are acid-Fast organisms Gram-positive or Gram- 2 Do your results indicate that endospores are acid-fast? Explain. 3 Why is it best to use "old" cultures for this staining procedure? 4 When you stain your Mycobacterium smegmatis, some of the cells are red and others are blue. What does tell you about your culture?
True (a) or false (b) 14. An acidic dye will bind more readily to the nucleic...
True (a) or false (b) 14. An acidic dye will bind more readily to the nucleic acids of a bacterial cell than a basic dye. d) 15. Bacterial cells divide by a process known as: a) meiosis b) mutation c)binary fission Osmosis 16-18. Match the following components of a stain with their correct descriptions A Benzene B. Ionachrome C. Auxochrome D. Chromophore 16. 17. 18. Conveys ionization to the colored component of the stain Colorless solvent of dye Colored component...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
6) The Gram stain will stain bacteria on the basis of the chemistry of the: a)...
6) The Gram stain will stain bacteria on the basis of the chemistry of the: a) plasma membranes b) chromosomes c) cell wall d cytoplasmic proteins e) ribosomes 7) The Acid-Fast stain will stain bacteria on the basis of the chemistry of the a) plasma membranes b) chromosomes c) cell wall d) cytoplasmic proteins e) ribosomes 8) The morphology or shape of bacteria that are streptococcal or staphylococcal is dependant of the bacterial: a) cell wall b) plasma membrane c)...
1. The most resistant of all non-spore-forming pathogens: a. S. pyogenes b. S. aureus c. S....
1. The most resistant of all non-spore-forming pathogens: a. S. pyogenes b. S. aureus c. S. pneumoniae d. N. gonorrhoeae e. N. meningitides 2. Pyogenic means: a. Fever-causing b. An extreme form of diarrhea c. Severe dehydration d. Pus-filled e. both a and d 3. Fill in the blank: This pathogen causes gas-gangrene: 4. The genus Clostridium is the causative agent of: a. Botulism b. Tetanus c. Colitis d. Gangrene e. all of the above 5. Bacillus is: a, Gram...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
• 7. This image shows results of a(n) stain: A. Spore; B. Acid Fast; C. Gram; D. Capsule 8. is the primary stain for this procedure. 9. Identify the organisms shown as to staining reaction and morphology.
MICROBIOLOGY:
Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after you have viewed your acid fast stain slides. Bacteria for this exercise are: Mycobacterium smegmatis (or M. phlei) and S. aureus. Q1. Acid fast bacteria are have some significant differences as compared to non-acid fast bacteria. What are the major chemical (structural) differences among these different bacteria that might explain the acid-fast stain? Hint: This structural difference also affects the way the bacteria grow...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
Questions What does the term "Acid Fa" mean? Are acid-Fast organisms Gram-positive or Gram- 2 Do your results indicate that endospores are acid-fast? Explain. 3 Why is it best to use "old" cultures for this staining procedure? 4 When you stain your Mycobacterium smegmatis, some of the cells are red and others are blue. What does tell you about your culture?
True (a) or false (b) 14. An acidic dye will bind more readily to the nucleic acids of a bacterial cell than a basic dye. d) 15. Bacterial cells divide by a process known as: a) meiosis b) mutation c)binary fission Osmosis 16-18. Match the following components of a stain with their correct descriptions A Benzene B. Ionachrome C. Auxochrome D. Chromophore 16. 17. 18. Conveys ionization to the colored component of the stain Colorless solvent of dye Colored component...
6) The Gram stain will stain bacteria on the basis of the chemistry of the: a) plasma membranes b) chromosomes c) cell wall d cytoplasmic proteins e) ribosomes 7) The Acid-Fast stain will stain bacteria on the basis of the chemistry of the a) plasma membranes b) chromosomes c) cell wall d) cytoplasmic proteins e) ribosomes 8) The morphology or shape of bacteria that are streptococcal or staphylococcal is dependant of the bacterial: a) cell wall b) plasma membrane c)...
1. The most resistant of all non-spore-forming pathogens: a. S. pyogenes b. S. aureus c. S. pneumoniae d. N. gonorrhoeae e. N. meningitides 2. Pyogenic means: a. Fever-causing b. An extreme form of diarrhea c. Severe dehydration d. Pus-filled e. both a and d 3. Fill in the blank: This pathogen causes gas-gangrene: 4. The genus Clostridium is the causative agent of: a. Botulism b. Tetanus c. Colitis d. Gangrene e. all of the above 5. Bacillus is: a, Gram...
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