In the acid-fast stain, the purpose of the methylene blue is to __________. (1) remove the...
In the acid-fast stain, the purpose of the methylene blue is to __________.
(1) remove the stain from non acid-fast cells
(2) kill bacteria
(3) stain non-acid-fast cells
(4) disinfect the slide
Homework Answers
The acid fast stain is a differential stain which distinuishes organisms with waxy cell wall that can resist decolorization with acid alcohol.
Acid alcohol decolorize the cells which decolorizes all cells except the acid fast ones.
Methylene blue is then applied to counterstain any cells which have been already decolorized. After the staining process, acid fast cells will be reddish - pink and non-acid fast cells will be blue.
thus in the acid fast stain, methylene blue is used to stain non-acid-fast cells.
Add Answer to:
In the acid-fast stain, the purpose of the methylene blue is to
__________.
(1) remove the...
MICROBIOLOGY: Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential...
MICROBIOLOGY:
Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
dyes, such as phenol red or methylene blue used in differential media? Why are dyes, such...
dyes, such as phenol red or methylene blue used in differential media? Why are dyes, such as A. to inhibit motility B. to stain microbes C to kill bacteria D. to see microbes to detect pH changes due to metabolism What is the purpose of differential medias to encourage growth of the desired bacteria B to isolate few bacteria to observe metabolism of the bacteria D. to stain bacteria E to determine motility 10 Why does Staphylococcus aureus turn yellow...
can methylene blue stain (simple stain) be used to determine if bacteria is anaerobic or aerobic?...
can methylene blue stain (simple stain) be used to determine if bacteria is anaerobic or aerobic? is there a another dye that may confirm this? thanks you.
Lab Report: 6 Acid-Fast Staining 1 What is the purpose of the acid-fast staining technique? To...
Lab Report: 6 Acid-Fast Staining 1 What is the purpose of the acid-fast staining technique? To Identity acil fast and on need fout bacteria To descrote the inhocal sigraf cance of acid-foot staining 2. Complete the following table with your observations Name of the Bacteria Color of the cells Acid-last/ non acid-last Drawing (color) 3. What might account for the fact that some bacteria are acid-fast and others are non acid-fast? What is the clinical significance of determining whether or...
why isn't the gram stain used on acid fast bacteria ?if you did Gram stain acid...
why isn't the gram stain used on acid fast bacteria ?if you did Gram stain acid fast bacteria ,what would their Gram reaction be?what is the gram reaction of non acid fast bacteria
Hi, LaurenSign OutA Home AB-Structural Stains croLab Tutor: Use and Application of the Acid-Fast Stain K2...
Hi, LaurenSign OutA Home AB-Structural Stains croLab Tutor: Use and Application of the Acid-Fast Stain K2 of 3 You INCORRECTLY perform an acid-fast stain on a smear from a mixed culture of Mycobacterium that you forget to use acid-alcohol during the procedure If you viewed this slide with a microscope smegmatis (acid-fast cells) and Staphylococcus aureus (non-acid-fast cells) Your mistake is what would each cell type look like? View Available Hint(s) M smegmatis cells would appear red S aureus cells...
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after...
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after you have viewed your acid fast stain slides. Bacteria for this exercise are: Mycobacterium smegmatis (or M. phlei) and S. aureus. Q1. Acid fast bacteria are have some significant differences as compared to non-acid fast bacteria. What are the major chemical (structural) differences among these different bacteria that might explain the acid-fast stain? Hint: This structural difference also affects the way the bacteria grow...
How do acid fast stain results compare to gram stain results? a. In other words, what...
How do acid fast stain results compare to gram stain results? a. In other words, what does the following represent? Acid Fast Stain (to Pink bacteria ACO fas广 Blue bacteria C AS Gram Stain Pink bacteria 6eAm- Violet bacteria GRAM f b. Why is there a difference in what the colors mean?
Lab Supplemental Questions hital Questions Acid-Fast Stain 1. Why is acid-alcohol used as the decoloriting agent...
Lab Supplemental Questions hital Questions Acid-Fast Stain 1. Why is acid-alcohol used as the decoloriting agent in the dio Aud-alcohol is used as the decolorizing Staining because it ensures that the pro form non acld fast organisme. agent in the acid-fast stain? orizing agent in the acid-fast the primary stain is removed 2. Why don't we Gram stain the mycobacterium? stain both acid-fast and non-acid-fast bacteria stain red, what 3. If at the end of the acid-fast stain both acid-fast...
MICROBIOLOGY:
Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...
dyes, such as phenol red or methylene blue used in differential media? Why are dyes, such as A. to inhibit motility B. to stain microbes C to kill bacteria D. to see microbes to detect pH changes due to metabolism What is the purpose of differential medias to encourage growth of the desired bacteria B to isolate few bacteria to observe metabolism of the bacteria D. to stain bacteria E to determine motility 10 Why does Staphylococcus aureus turn yellow...
Lab Report: 6 Acid-Fast Staining 1 What is the purpose of the acid-fast staining technique? To Identity acil fast and on need fout bacteria To descrote the inhocal sigraf cance of acid-foot staining 2. Complete the following table with your observations Name of the Bacteria Color of the cells Acid-last/ non acid-last Drawing (color) 3. What might account for the fact that some bacteria are acid-fast and others are non acid-fast? What is the clinical significance of determining whether or...
Hi, LaurenSign OutA Home AB-Structural Stains croLab Tutor: Use and Application of the Acid-Fast Stain K2 of 3 You INCORRECTLY perform an acid-fast stain on a smear from a mixed culture of Mycobacterium that you forget to use acid-alcohol during the procedure If you viewed this slide with a microscope smegmatis (acid-fast cells) and Staphylococcus aureus (non-acid-fast cells) Your mistake is what would each cell type look like? View Available Hint(s) M smegmatis cells would appear red S aureus cells...
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after you have viewed your acid fast stain slides. Bacteria for this exercise are: Mycobacterium smegmatis (or M. phlei) and S. aureus. Q1. Acid fast bacteria are have some significant differences as compared to non-acid fast bacteria. What are the major chemical (structural) differences among these different bacteria that might explain the acid-fast stain? Hint: This structural difference also affects the way the bacteria grow...
How do acid fast stain results compare to gram stain results? a. In other words, what does the following represent? Acid Fast Stain (to Pink bacteria ACO fas广 Blue bacteria C AS Gram Stain Pink bacteria 6eAm- Violet bacteria GRAM f b. Why is there a difference in what the colors mean?
Lab Supplemental Questions hital Questions Acid-Fast Stain 1. Why is acid-alcohol used as the decoloriting agent in the dio Aud-alcohol is used as the decolorizing Staining because it ensures that the pro form non acld fast organisme. agent in the acid-fast stain? orizing agent in the acid-fast the primary stain is removed 2. Why don't we Gram stain the mycobacterium? stain both acid-fast and non-acid-fast bacteria stain red, what 3. If at the end of the acid-fast stain both acid-fast...
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