1.
. To determine if the bacteria contain a waxy material, mycolic acid in their cell walls which is resistant to your immune system.
. To determine the clinical significance of acid-fast staining by identifying acid-fast and non-acid fast bacteria.
2.
3. Bacteria that contain mycolic acid show positive result and bacteria does not contain mycolic acid show negative result.
Mycolic acids are glycolipids that shows poor absorption followed by high retention and is found in the waxy cell walls of a group of bacteria of Mycolata taxon such as Mycobacterium tuberculosis, M. leprae etc.
4. The acid-fast stain can determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.
A negative result for an acid-fast bacteria smear means there are no bacteria were found in the sputum sample. A positive result implies that the bacteria were found and may the person have an infection.
Lab Report: 6 Acid-Fast Staining 1 What is the purpose of the acid-fast staining technique? To...
1. There are other staining procedures beyond simple staining and differential staining. Examples include endospore, acid-fast, capsule, and flagella stains. Please pick one staining technique to research and answer the following questions: • What is the theory behind the staining technique, what stains are used, how are they applied, what portion of the bacteria are they reacting with • How is this stain used to differentiate or identify bacterial cells or cell structures? • Describe the staining technique. List and...
What is the purpose of the heat-fix step in the direct staining technique? A heat allows the cells to absorb the stain O B cells attach to the slide с cells separate creating a monolayer D this step kills bacteria
Acid Fast Staining Carbolfuchsin Acid-Alcohol Q Methylene Blue Using the reagents above and the cells below. Show the steps in Acid Fast staining by drawing arrows and reagents in their proper places. I will start the first step for you. Show the end result of Acid Fast Staining for Acid Fast+ and Acid Fast-cells. A. Acid Fast Positive Step 1. Add Carbolfuchsin B. Acid Fast Negative Step 1. Add Carbolfuchsin Step 2. Add Step 2. Add (make sure you show...
In the acid-fast stain, the purpose of the methylene blue is to __________. (1) remove the stain from non acid-fast cells (2) kill bacteria (3) stain non-acid-fast cells (4) disinfect the slide
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
Lab Supplemental Questions hital Questions Acid-Fast Stain 1. Why is acid-alcohol used as the decoloriting agent in the dio Aud-alcohol is used as the decolorizing Staining because it ensures that the pro form non acld fast organisme. agent in the acid-fast stain? orizing agent in the acid-fast the primary stain is removed 2. Why don't we Gram stain the mycobacterium? stain both acid-fast and non-acid-fast bacteria stain red, what 3. If at the end of the acid-fast stain both acid-fast...
QUESTIONS 1) Are acid fast cells gram positive or gram negative? Explain why it might be difficult to determine the Gram reaction of acid fast cells. 2) What is the significance of the mycolic acid layer in the following contexts? A) To bacteria for survival and infection B) During the acid fast staining procedure QUESTIONS 1) What is the difference between a pure culture and a pure colony? Is a streaking for isolation plate a pure culture when your original...
lake-up Micro Lab: Identifying Unknown bacteria: dentify the Unknown Bacteria Lab Experiment elow are results from the unknown lab experiment. The identity of this bacteria is not known. Your ask is to use the data below to form a hypothesis of the identity of this bacteria. Record your results on he chart (Table 1) below and make a prediction on the bacteria based on your results. There is a list of ossible bacteria below. You may need to look back...
Lab Report-Carbohydrates 1. Purpose 2. Special Media for Isolating Bacteria (Lab #12) a. Why are dyes such as phenol red, eosin or methylene blue added to the media? b. How does the bacterium change the media (i.e color of agar or colonies) after incubation? C. In this experiment, which media are selective, and which are differential? d. How did the results observe on the mannitol salt agar and EMB agar correlate to the Gram reaction of the bacteria? e. What...
MICROBIOLOGY: Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...