Question

lake-up Micro Lab: Identifying Unknown bacteria: dentify the Unknown Bacteria Lab Experiment elow are results from the unknown lab experiment. The identity of this bacteria is not known. Your ask is to use the data below to form a hypothesis of the identity of this bacteria. Record your results on he chart (Table 1) below and make a prediction on the bacteria based on your results. There is a list of ossible bacteria below. You may need to look back at your notes or use the internet to confirm your esults. This completed chart will need to be reproduce for your lab summary. Number on Tube Morphology Gram Stain Results Endospore Results Acid Fast Guess the name of the bacteria non Ifacultative anaerobe o l It coli o Jacid fast List of possible unknown bacteria: Escherichia coli Bacillus cereus Salmonella typhimurium Mycobacterium smegmatis Vibrio fischeri Staphylococcus epidermidis Micrococcus luteus

Answer 1

Escherichia coli

Gram-negative

Non acid fast

vegetative cell

1.Acid fast test

The surface of cells and cytoplasm is negatively charged, will the positively charged stains adhere readily to the cell surface.

2.

ACID-FAST STAIN		Cell Color	Cell Color
Procedure	Reagent	Acid-fast Bacteria	Nonacid-fast Bacteria
Primary dye	Carbolfuchsin	red	red
Decolorizer	Acid-alcohol	red	colorless
Counterstain	Methylene blue	red	blue