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in the Micro lab, I got an unknown microorganism. The assignment is to find the microorganism...

in the Micro lab, I got an unknown microorganism. The assignment is to find the microorganism by performing various tests on it.

My hypothesis is: Unknown organism is virulent and can cause diseases in the human organism.

My question: Which 4 test should I do first to detect my organism and why those tests in particular? (I am thinking aerotolerance, endospore, hemolysis...)

depending on each test result can you give an example of the microorganism that it can be?

Colony Morphology  

Acid-fast Stain  

Endospore Stain  

Gram Stain

Aerotolerance  

Catalase  

EMB  

Hemolysis    

Mannitol Salt Agar  

Motility Test Medium

MR-VP

PR Glucose

PR Lactose

PR Sucrose

Starch Hydrolysis

TSI

Urea Hydrolysis

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Answer #1

The correct options are-

1. Hemolysis--hemolysis test will indicate wthether the bacteria is able to cause lysis of the blood cells

2. TSI test--it is used to identify the enteric pathogens

3. Colony morphology--is used to identify the pathogen

4. Endospore stain--spore forming bacteria are the most pathogenic types-because they escape the physical immune defecnce mechanisms in the body and can efficiently cause disease.

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