Homework Answers
1.a)The first test that i would like to do is Gram staining.
Gram staining shows the morphology of the bacteria i.e., cocci or bacilli and also if the organism is gram +ve or gram -ve.
b) The result of the test- The organism is gram +ve cocci.
Yes, Catalase test will be helpful for determining the unknown.
Catalase test will help to differentiate if the gram +ve cocci is Staphylococcus or Streptococcus. Staphylococcus is Gram +ve and streptococcus is Gram -ve.
c) The result is- Catalase +ve. So the genus of the organism is Staphylococcus.
d) The sheep blood agar shows complete hemolysis i.e., Beta type of hemolysis. So the unknown organism will be - Staphylococcus aureus.
There are 3 types of hemolysis.
1.Alpha (a ) -hemolysis: incomplete lysis of RBC - Greenish, cloudy zone around the colony. Example- Streptococcus pneumoniae.
2. Beta ( b)-hemolysis: complete lysis of RBC - A clear zone with a clean edge around the colony. Example-Staphylococcus aureus.
3. Gamma ( g)-hemolysis: no lysis of RBC - No change in the blood agar around the colony. Example- Staphylococcus epidermidis.
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mixed unknown. the goal of this assignment is to identity each
organism. so this attachement or picture has both organism
seperated by gram stanin and result of each on macConkeys Agar and
Columbia CNA. so in order to identify the genus and specise both
organsims, i have to order 2 additional test per. MY questions to
you is what kinds of test do have to requesd or do in oder to
identity the organism ?
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