Homework Answers
Ans.
The correct answer is (D) this step kills bacteria
Explanation:
In direct staining techniques,it is very necessary that the bacterial smear is firmly adhered to the slide so that cells can absorb the stain properly and accurate result can be obtained.This is the reason why heat fixing is done before staining the bacteria.Heat fixing step kills the bacteria in the smear and helps it to firmly adhere to the slide,so that the sample can take up the stains more readily.Heat helps the bacterial proteins to coagulate and in effectively killing the bacteria so that they can adhere to the slide properly.
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What is the purpose of the heat-fix step in the direct staining technique? A heat allows...
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A common first step in staining procedures such as the Gram stain is to prepare a heat-fixed bacterial smear. Why is this technique NOT the best for accurately detemining the size of the bacterial cell? a. the heat will prevent ethanol from dissolving the inner membrane and makes it hard to decipher cell size b. the heat will enhance the formation of crystal violet-iodine complexes, which will make the bacteria appear larger than they actually are c. the heat fixes...
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all questions
ASSESSMENT Critical Thinking and Learning Outcomes Review 1. What are the two purposes of heat fixation? 2. What is the purpose of simple staining? 3. Why are basic dyes more successful in staining bacteria than acidic dyes? 4. Name three basic stains 5. Why is time an important factor in simple staining? 6. How would you define a properly prepared bacterial smear? 7. Why should you use an inoculating needle when making smears from solid media? An inoculating...
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Lab Report: 6 Acid-Fast Staining 1 What is the purpose of the acid-fast staining technique? To Identity acil fast and on need fout bacteria To descrote the inhocal sigraf cance of acid-foot staining 2. Complete the following table with your observations Name of the Bacteria Color of the cells Acid-last/ non acid-last Drawing (color) 3. What might account for the fact that some bacteria are acid-fast and others are non acid-fast? What is the clinical significance of determining whether or...
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the purpose of each of the following reagents in a differential staining procedure: a. Primary stain b. Counter stain c. Decolorizing agent d. Mordant 3. Why is it important for the counter stain to be a lighter color than the primary stain? 4. You have done gram staining for a gram positive bacteria, but at the end you observed pinkish-red bacteria under microscope, what was...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. Define acidic and basic dyes. What is the purpose of each? 2. What is the purpose of fixing a slide that is to be stained? 3. Why are specimens to be stained suspended in sterile saline or distilled water? 4. Which of the microscope objectives is most satisfactory for studying bacteria?...
all questions
ASSESSMENT Critical Thinking and Learning Outcomes Review 1. What are the two purposes of heat fixation? 2. What is the purpose of simple staining? 3. Why are basic dyes more successful in staining bacteria than acidic dyes? 4. Name three basic stains 5. Why is time an important factor in simple staining? 6. How would you define a properly prepared bacterial smear? 7. Why should you use an inoculating needle when making smears from solid media? An inoculating...
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adım sua LISNA SPECIAL MATCHING Match only within the horizontal lines. Match the items on the right with the termsstatements questions on the left to give the one best answer. Use answers only once. Use all. 54. White colonies, G-) rods in chains 2. Bacitus 55. Pink colonies, G-) rods, irregular b. Mycobacterium 56. Yellow colonies, G(+) cocci in tetrads C. E. coli 57. Endospore forming, G(+) rods in chains d. Serratia 58. Acid fast, G(+) rods, irregular Micrococcus luteus...
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