Question 1: acidic dyes are referred to as the dyes that would be having a negative charge such that these would be able to bind to the positive structures present in the cell. These re used less often intne microbiology lab. An example of an acidic stain is the capsule staining, which would be staining the background. Examples of stain are India ink, acid fuschin, picnic acid as well as nigrosine.
Basic dyes are the ones that would be having a positive charge and would be binding to molecules that are negatively charged. These molecules would be nucleus acids. These are used more commonly since, the bacterial surfaces are negatively charged, due to the presence of teichoic acid. These are used more commonly. Examples are safranin, methylene blue, crystal violet or basic fuschin.
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and...
all questions ASSESSMENT Critical Thinking and Learning Outcomes Review 1. What are the two purposes of heat fixation? 2. What is the purpose of simple staining? 3. Why are basic dyes more successful in staining bacteria than acidic dyes? 4. Name three basic stains 5. Why is time an important factor in simple staining? 6. How would you define a properly prepared bacterial smear? 7. Why should you use an inoculating needle when making smears from solid media? An inoculating...
Effectiveness of Disinfectants Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. Answer the first 9 questions True or False 1. Disinfectants always remove all bacteria and fungal spores from an object. Antiseptic is another term for disinfectant. The presence of blood serum, skin tissue and other body fluids would interfere with the antimicrobial action of disinfectants. The use of carbolic acid as...
Differential Tests Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. What is the color of phenoi red at an acid pH? Yellow Cola 2. What is the function of a Durham tube? 3. Name one indole positive organism. E.coli 4. Name two methods for determining bacterial motility. 5. Why is it essential to have pure cultures for biochemical tests? 6. Could...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
A sentence or two stating the hypothesis or hypotheses made. A sentence or two describing what was done A summary of the most important results A sentence stating the major conclusion EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...
100. Flare is: (a) the widest frequency of light that can enter an objective (b) the bending of light as it passes through different substances (c) wavelength/2NA (d) when the beam of light "spreads" wider than the objective's "hole" (e) wavelengths of light too short to enter the objective 101. Coccobacillus shaped bacteria are considered to be_?_ (a) cocci (b) bacilli (c) diplobacilli (d) diplococci (e)answers a and b only 102. This microscope is used for cheap and quick "'diagnosis"...
what are the possible question and answers for the following topic? Questions will be distributed approximately as follows: Substance Use Disorder and Cannabis - 10 Pain Management –20 Physiologic Stress and Pharmacologic Steroids- 20 Cumulative material – 2-3 questions from each of the preceding weeks. Exam questions come from class lectures, slides, readings and media resources. Approximately 2/3 of exam questions are knowledge base questions, and approximately 1/3 are application questions that ask you to apply what you have learned....