can methylene blue stain (simple stain) be used to
determine if bacteria is anaerobic or aerobic?
is there a another dye that may confirm this?
thanks you.
Yes, methylene blue which is a simple stain can be used to determine whether the bacteria in our culture medium is aerobic or anaerobic.
Resazuin is also a redox indicator that can be used in the laboratory apart from methylene blue.
Advantages of using methylene blue:
1. It is cheap.
2. Easily accessible dye.
3. Widely used in microbiological laboratories
4. Results obtained with this dye will be more stable over time.
can methylene blue stain (simple stain) be used to determine if bacteria is anaerobic or aerobic?...
dyes, such as phenol red or methylene blue used in differential media? Why are dyes, such as A. to inhibit motility B. to stain microbes C to kill bacteria D. to see microbes to detect pH changes due to metabolism What is the purpose of differential medias to encourage growth of the desired bacteria B to isolate few bacteria to observe metabolism of the bacteria D. to stain bacteria E to determine motility 10 Why does Staphylococcus aureus turn yellow...
In the acid-fast stain, the purpose of the methylene blue is to __________. (1) remove the stain from non acid-fast cells (2) kill bacteria (3) stain non-acid-fast cells (4) disinfect the slide
45. Which detection method would be more useful for observing fungus rather bacteria? a. Simple stain b. Gram stain c. Endospore stain d. Negative stain e. Wet mount Match the following: A) Crystal violet B) Methylene blue C) Malachite green D) Safranin E) Iodine 46. Acts as the primary stain in the simple stain procedure. 47. Acts as the counterstain in the Gram stain procedure 48. Acts as the primary stain in the Gram stain procedure 49. Used to stain...
2. If after incubation, you observed that the methylene blue indicator strip inside the jar was blue, what would you guess the normal environment to be- aerobic or anaerobic? How would you expect the growth on the plate inside the jar to differ from the plate incubated outside the jar?
BI0S242, Week 7, Lab 1 2. Do all the bacteria samples grow on Eosin Methylene Blue and MacConkey agar? not, why What does a metallic green sheen indicate on an Eosin Methylene Bhue plate? 3. 4. What additional information did the lactose fermentation tubes provide? Why couldn't we examine any anaerobic bacteria that are part of the normal digestive system? 5. 6. Were there any colonies in the rest room samples? Could you identify any that gave similar results on...
In the Gram Stain, safranin is the dye used to stain gram negative bacteria. This solution is 0.5%(wt/vol) safranin. How many milligrams of safranin are in a 120 mL safranin solution? Report your answer to two decimal places. Perform all calculations in a single step using Excel to avoid accruing rounding error.
help please What type(s) of bacteria are favored to grow on eosin methylene blue (EMB) agar? Select one: O a Gram positive bacteria that can undergo anaerobic respiration. O b. Gram negative bacteria that can ferment glucose and/ or lactose, O c. Only those bacilli that can utilize citrate as a main carbohydrate source. O d. All Bacteria would grow equally well A bacterial species grows on a MacConkey agar plate, and the colonies have dark red color with hazy...
based on chromatographic methods lab which dye ( methylene blue and methylene orange) is more polor ? how did you distinguish between chlorophyll a nad chlorophyll b in your TLC analysis ? which structural feature of the molecule accounts for this observation ? compare and contrst your TLC results whe n100% acetone was used vs when 100% hexane was used what do your result suggest the polarities of the dyes in spinach ?
what three characteristics of the bacteria corynebacterium are you supposed to see in the simple stain slide you prepared
why isn't the gram stain used on acid fast bacteria ?if you did Gram stain acid fast bacteria ,what would their Gram reaction be?what is the gram reaction of non acid fast bacteria