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can methylene blue stain (simple stain) be used to determine if bacteria is anaerobic or aerobic?...

can methylene blue stain (simple stain) be used to determine if bacteria is anaerobic or aerobic?
is there a another dye that may confirm this?
thanks you.

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Answer #1

Yes, methylene blue which is a simple stain can be used to determine whether the bacteria in our culture medium is aerobic or anaerobic.

Resazuin is also a redox indicator that can be used in the laboratory apart from methylene blue.

Advantages of using methylene blue:

1. It is cheap.

2. Easily accessible dye.

3. Widely used in microbiological laboratories

4. Results obtained with this dye will be more stable over time.

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