0.5% means 0.5 gram in 100 ml
So in 1 ml safranin would be 0.5/100 = 0.005 gram
So for extra 20 ml safranin required = 0.005 * 20 =0.1 gram
So total safranin required for 120 ml = 0.5 + 0.1 = 0.6 grams = 600 mg
In the Gram Stain, safranin is the dye used to stain gram negative bacteria. This solution...
When performing the gram stain, the the dye safranin is suspended in a 95% ethanol solution. If only 96% ethanol is available, how many mL of ethanol should be added to achieve a final volume of 25 with the correct percent ethanol? Report your answer to 2 decimal places. Perform all calculations in a single step using Excel to avoid accumulated rounding error.
In the gram stain, crystal violet is used as the primary dye and safranin as the counterstain. Can these dyes be replaced by other dyes without affecting the main function? And if the answer is yes, what dyes can be substituted?
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
Microbiologists use acid-alcohol when performing a cell stain of the pathogen Mycobacterium tuberculosis. A common recipe is to make 3%(wt/vol) HCl in ethanol. HC has a formula weight of 36.46 grams per mole. If the stock solution of HCl is 1 moles per 1000 ml, how many mL of HCI need to be added to achieve a final volume of 100 mL of acid alcohol solution? Report your answer to two decimal places. Algebra can be used to solve this...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
BACKGROUND: Mycobacterium tuberculosis causes tuberculosis, one of the world’s deadliest diseases. It is an acid-fast bacillus that is 2-4 um in length and 0.2-0.5 um in width. How many weeks, days,& hours would it take to see a M.tuberculosis colony on solid media? Its generation time is listed in the reading (above). Assume that bacterial colonies are not visible to the naked eye unless they contain ≥ 2 millioncells. This species forms aggregates of 5 cells on average. Show your...
A sentence or two stating the hypothesis or hypotheses made. A sentence or two describing what was done A summary of the most important results A sentence stating the major conclusion EXPERIMENT 2: CHARACTERIZING BACTERIA ISOLATED FROM SOIL Data Tables Table 1: Gram Staining Observations Sample Colony 1 Colony 2 Colony 3 + "Normal" + "Heated" Table 2: Experiment 2b Catalase Assay Results Colony Catalase Assay Results ("Normal") Catalase Assay Results ("Heated') 1 2 3 + Characterizing Bacteria Isolated from...
microbiology help TOT Zoo Add Page Insert Table Chart Text Shape Media Comment These questions will serve in lieu of a lab report for Exercise 15, 16, and 17 You will find the answer to these questions in the background, procedure, results and interpretation sections of manual Exercise 15, 16, and 17, videos, Actions of Selective and Differential Media Chart, and the Principle/Theory article in homework section.) General Questions 1. What is the purpose (function) of selective media? (How does...
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCl stock solution. In your response to this question, be very specific about the quantities of stock solution and deionized water to be used in the dilution and the...
100. Flare is: (a) the widest frequency of light that can enter an objective (b) the bending of light as it passes through different substances (c) wavelength/2NA (d) when the beam of light "spreads" wider than the objective's "hole" (e) wavelengths of light too short to enter the objective 101. Coccobacillus shaped bacteria are considered to be_?_ (a) cocci (b) bacilli (c) diplobacilli (d) diplococci (e)answers a and b only 102. This microscope is used for cheap and quick "'diagnosis"...