In the gram stain, crystal violet is used as the primary dye and safranin as the...
In the gram stain, crystal violet is used as the primary dye and
safranin as the counterstain. Can these dyes be replaced by other
dyes without affecting the main function? And if the answer is yes,
what dyes can be substituted?
Homework Answers
Yes, you can replaced by other dyes without affecting the main function of primary stain and counter stain as crystal violate and Safranin.
In the gram staining, crystal violate is a primary dye that used first in the gram staining process. Methylene blue is also a primary dye and it may used as alternative of crystal violate.
In the other hand, after decolonization Safranin is used in gram staining as a counter stain. The main function of Safranin is Colourized in pink colour in the decolourized cells. Basic fuchsin is a stain used as alternative of Safranin or counter stain. It is also give the decolourized cells as pink colour.
If any question, please comment.
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In the gram stain, crystal violet is used as the primary dye and
safranin as the...
In the Gram Stain, safranin is the dye used to stain gram negative bacteria. This solution...
In the Gram Stain, safranin is the dye used to stain gram negative bacteria. This solution is 0.5%(wt/vol) safranin. How many milligrams of safranin are in a 120 mL safranin solution? Report your answer to two decimal places. Perform all calculations in a single step using Excel to avoid accruing rounding error.
what dyes are use as the primary stain and the counterstain in Gram- staining?
what dyes are use as the primary stain and the counterstain in Gram- staining?
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin,...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
45. Which detection method would be more useful for observing fungus rather bacteria? a. Simple stain...
45. Which detection method would be more useful for observing fungus rather bacteria? a. Simple stain b. Gram stain c. Endospore stain d. Negative stain e. Wet mount Match the following: A) Crystal violet B) Methylene blue C) Malachite green D) Safranin E) Iodine 46. Acts as the primary stain in the simple stain procedure. 47. Acts as the counterstain in the Gram stain procedure 48. Acts as the primary stain in the Gram stain procedure 49. Used to stain...
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When performing the gram stain, the the dye safranin is suspended in a 95% ethanol solution....
When performing the gram stain, the the dye safranin is suspended in a 95% ethanol solution. If only 96% ethanol is available, how many mL of ethanol should be added to achieve a final volume of 25 with the correct percent ethanol? Report your answer to 2 decimal places. Perform all calculations in a single step using Excel to avoid accumulated rounding error.
Gram Staining Safranin O lodine | Crystal Violet | Decolorizer Using the reagents above and the...
Gram Staining Safranin O lodine | Crystal Violet | Decolorizer Using the reagents above and the cells below. Show the steps in Gram staining by drawing arrows and reagents in their proper places. I will start the first step for you. Show the end result of Gram Staining for G+ and G cells. A. Gram Positive B. Gram Negative Step 1: Add Crystal Violet Step 1: Add Crystal Violet Step 2: Add_ Step 2: Add (make sure you show what...
QUESTION 15 Why is crystal violet used in the Gram stain retained in the cell wall...
QUESTION 15 Why is crystal violet used in the Gram stain retained in the cell wall of Gram positive bacteria, but not in Gram negative bacteria? Gram positive bacteria have a thick peptidoglycan layer while Gram negative bacteria have a thin peptidoglycan layer. Gram positive bacteria form spores and Gram negative do not. O Gram positive bacteria have no peptidoglycan layer and Gram negative bacteria have a thick cell wall O Gram positive bacteria have capsules and Gram negative bacteria...
Can you please explain why SAFRANIN cannot be used a primary stain, when staining Bacillus Cereus...
Can you please explain why SAFRANIN cannot be used a primary stain, when staining Bacillus Cereus and Clostridium tetani?
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
In the Gram Stain, safranin is the dye used to stain gram negative bacteria. This solution is 0.5%(wt/vol) safranin. How many milligrams of safranin are in a 120 mL safranin solution? Report your answer to two decimal places. Perform all calculations in a single step using Excel to avoid accruing rounding error.
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
45. Which detection method would be more useful for observing fungus rather bacteria? a. Simple stain b. Gram stain c. Endospore stain d. Negative stain e. Wet mount Match the following: A) Crystal violet B) Methylene blue C) Malachite green D) Safranin E) Iodine 46. Acts as the primary stain in the simple stain procedure. 47. Acts as the counterstain in the Gram stain procedure 48. Acts as the primary stain in the Gram stain procedure 49. Used to stain...
Question 7 1.25 pts Crystal violet is used for? (choose all that apply) Stain to distinguish Gram -/+ bacteria Disinfectant pH indicator Hair, fabric dye Ingredient in paints and ink Question 8 1.25 pts Indigo is used for (choose all that apply): Ingredient in paints Food coloring Fabric dye organic semiconductor
When performing the gram stain, the the dye safranin is suspended in a 95% ethanol solution. If only 96% ethanol is available, how many mL of ethanol should be added to achieve a final volume of 25 with the correct percent ethanol? Report your answer to 2 decimal places. Perform all calculations in a single step using Excel to avoid accumulated rounding error.
Gram Staining Safranin O lodine | Crystal Violet | Decolorizer Using the reagents above and the cells below. Show the steps in Gram staining by drawing arrows and reagents in their proper places. I will start the first step for you. Show the end result of Gram Staining for G+ and G cells. A. Gram Positive B. Gram Negative Step 1: Add Crystal Violet Step 1: Add Crystal Violet Step 2: Add_ Step 2: Add (make sure you show what...
QUESTION 15 Why is crystal violet used in the Gram stain retained in the cell wall of Gram positive bacteria, but not in Gram negative bacteria? Gram positive bacteria have a thick peptidoglycan layer while Gram negative bacteria have a thin peptidoglycan layer. Gram positive bacteria form spores and Gram negative do not. O Gram positive bacteria have no peptidoglycan layer and Gram negative bacteria have a thick cell wall O Gram positive bacteria have capsules and Gram negative bacteria...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
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