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In the gram stain, crystal violet is used as the primary dye and safranin as the...


In the gram stain, crystal violet is used as the primary dye and safranin as the counterstain. Can these dyes be replaced by other dyes without affecting the main function? And if the answer is yes, what dyes can be substituted?

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Answer #1

Yes, you can replaced by other dyes without affecting the main function of primary stain and counter stain as crystal violate and Safranin.

In the gram staining, crystal violate is a primary dye that used first in the gram staining process. Methylene blue is also a primary dye and it may used as alternative of crystal violate.

In the other hand, after decolonization Safranin is used in gram staining as a counter stain. The main function of Safranin is Colourized in pink colour in the decolourized cells. Basic fuchsin is a stain used as alternative of Safranin or counter stain. It is also give the decolourized cells as pink colour.

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