The dyes that are used as the primary stain and the counterstain in Gram- staining;
The primary stain; the crystal violet.
Counterstain; of basic fuchsin (Gives the decolorized bacteria a pink color).
what dyes are use as the primary stain and the counterstain in Gram- staining?
In the gram stain, crystal violet is used as the primary dye and safranin as the counterstain. Can these dyes be replaced by other dyes without affecting the main function? And if the answer is yes, what dyes can be substituted?
Exercise 8 - Gram Staining Procedure for gram staining. Know all chemicals/reagents used in correct order, function of each chemical, the effect of cach chemical and how gram + and gram - bacteria would appear at each step Color reactions at each step of procedure. Know why, based on the differences in cell wall structure, that the gram + vs gram - bacteria stain either purple or pink. What washes off in the decolorizing step of a gram - bacteria?...
all questions ASSESSMENT Critical Thinking and Learning Outcomes Review 1. What are the two purposes of heat fixation? 2. What is the purpose of simple staining? 3. Why are basic dyes more successful in staining bacteria than acidic dyes? 4. Name three basic stains 5. Why is time an important factor in simple staining? 6. How would you define a properly prepared bacterial smear? 7. Why should you use an inoculating needle when making smears from solid media? An inoculating...
DIFFERENTIAL STAINING 1. Primary Stain- what structure is stained? 2. Mordant- what is this?
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the purpose of each of the following reagents in a differential staining procedure: a. Primary stain b. Counter stain c. Decolorizing agent d. Mordant 3. Why is it important for the counter stain to be a lighter color than the primary stain? 4. You have done gram staining for a gram positive bacteria, but at the end you observed pinkish-red bacteria under microscope, what was...
Review your knowledge of the Gram-staining protocol; then, using the following information, construct a new Gram-staining protocol. Hasmid Dark-Green stain will stain any cell type Jussin Orange stain will stain any cell type Lykin stain will complex with Hasmid Dark-Green to generate a larger compound Rank the options below:(1-5) Stain cells with Hasmid Dark-Green stain followed by a rinse with water Blot dry Stain with Jussin Orange stain followed by a rinse with water Decolorize with alcohol followed by a...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
• 7. This image shows results of a(n) stain: A. Spore; B. Acid Fast; C. Gram; D. Capsule 8. is the primary stain for this procedure. 9. Identify the organisms shown as to staining reaction and morphology.
A common first step in staining procedures such as the Gram stain is to prepare a heat-fixed bacterial smear. Why is this technique NOT the best for accurately detemining the size of the bacterial cell? a. the heat will prevent ethanol from dissolving the inner membrane and makes it hard to decipher cell size b. the heat will enhance the formation of crystal violet-iodine complexes, which will make the bacteria appear larger than they actually are c. the heat fixes...
• 7. This image shows results of a(n). stain: A. Spore; B. Acid Fast; C. Gram; D. Capsule 8. is the primary stain for this procedure. • 9. Identify the organisms shown as to staining reaction and morphology. Show transcribed image text