Question

Review your knowledge of the Gram-staining protocol; then, using the following information, construct a new Gram-staining protocol.

• Hasmid Dark-Green stain will stain any cell type
• Jussin Orange stain will stain any cell type
• Lykin stain will complex with Hasmid Dark-Green to generate a larger compound

Rank the options below:(1-5)

• Stain cells with Hasmid Dark-Green stain followed by a rinse with water

• Blot dry

• Stain with Jussin Orange stain followed by a rinse with water

• Decolorize with alcohol followed by a rinse with water

• Stain cells with Lykin stain followed by a rinse with water

Answer 1

Options ranked below,

1. Stain cells with Hasmid Dark- Green stain - primary stain which will colour the bacteria followed by rinsing with water

2. Stain cells with Lykin stain who will form complex with Hasmid Dark to generate a larger compound. It act as a mordent who fixes the primary stain on bacteria

3. Decolorise with alcohol followed by a rinse with water and drain completely

4. Stain cells with Jussin Orange stain - secondary stain

5 Blot dry and observe