Can you please explain why SAFRANIN cannot be used a primary stain, when staining Bacillus Cereus...
Can you please explain why SAFRANIN cannot be used a primary stain, when staining Bacillus Cereus and Clostridium tetani?
Homework Answers
Sol: SAFRANIN cannot be used as a primary stain while staining Bacillus cereus and Clostridium tetani because while staining these bacteria we cant differentiate between gram positive or gram negative clearly as both the bacteria will retain the secondary stain.
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Can you please explain why SAFRANIN cannot be used a primary
stain, when staining Bacillus Cereus...
In the gram stain, crystal violet is used as the primary dye and safranin as the...
In the gram stain, crystal violet is used as the primary dye and safranin as the counterstain. Can these dyes be replaced by other dyes without affecting the main function? And if the answer is yes, what dyes can be substituted?
NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. Define acidic and basic dyes. What is the purpose of each? 2. What is the purpose of fixing a slide that is to be stained? 3. Why are specimens to be stained suspended in sterile saline or distilled water? 4. Which of the microscope objectives is most satisfactory for studying bacteria?...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
Explain why, when auditing to estimate a population total amount, a Z-test cannot be used.
Explain why, when auditing to estimate a population total amount, a Z-test cannot be used.
And can you please explain why? 21. Which is not one of the limitations usually associated...
And can you please explain why?
21. Which is not one of the limitations usually associated with the Friedel-Crafts alkylation reaction. a. Reaction fails when strongly deactivating substituents are present. b. Rearrangements of the carbocation intermediate are possible; n-alkyl derivatives are unavailable. c. Multiple alkylations frequently occur. d. Long branched chains of carbons cannot be used. e. An amine nitrogen cannot be present on the ring.
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT...
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
1- Explain, as best you can, why the primary key of the "one" side becomes the...
1- Explain, as best you can, why the primary key of the "one" side becomes the foreign key on the "many" side in a 1:M relationship?
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
Can someone help me i have to write an Final Essays about this paper. 1. Please...
Can someone help me i have to write an Final Essays about this paper. 1. Please discuss the mechanisms that Clostridium tetani and botulinum, Streptococcus pneumonia and Trypanosome cruz and bruce. utilize to cause disease in the nervous system. You must state what disease these microorganisms cause . in addition, please discuss that host immune defenses that the microbes would have to overcome to be success fuel at causing disease (hint:) A. include the three routes of entry to gain...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. Define acidic and basic dyes. What is the purpose of each? 2. What is the purpose of fixing a slide that is to be stained? 3. Why are specimens to be stained suspended in sterile saline or distilled water? 4. Which of the microscope objectives is most satisfactory for studying bacteria?...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
Explain why, when auditing to estimate a population total amount, a Z-test cannot be used.
And can you please explain why?
21. Which is not one of the limitations usually associated with the Friedel-Crafts alkylation reaction. a. Reaction fails when strongly deactivating substituents are present. b. Rearrangements of the carbocation intermediate are possible; n-alkyl derivatives are unavailable. c. Multiple alkylations frequently occur. d. Long branched chains of carbons cannot be used. e. An amine nitrogen cannot be present on the ring.
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
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