Homework Answers
a. Gram positive bacteria have a thick peptidoglycan layer while Gram negative bacteria have a thin peptidoglycan layer
Gram positive bacteria possess a thick peptidoglycan layer which retains the crystal violet stain whereas the Gram negative bacteria possess a thin peptidoglycan layer which is not able to retain the crystal violet stain.
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QUESTION 15 Why is crystal violet used in the Gram stain retained in the cell wall...
30. PRINCIPLES OF BIOLOGY BIOL 1456 Bacteria that retain crystal violet stain differ from those that...
30. PRINCIPLES OF BIOLOGY BIOL 1456 Bacteria that retain crystal violet stain differ from those that do not retain the stain in having a a. thick lipoprotein layer. c. thicker peptidoglycan layer. b. thick lipopolysaccharide layer. o d cell wall.
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
In the gram stain, crystal violet is used as the primary dye and safranin as the...
In the gram stain, crystal violet is used as the primary dye and safranin as the counterstain. Can these dyes be replaced by other dyes without affecting the main function? And if the answer is yes, what dyes can be substituted?
Cell Structure and Function: 1. What is the basic structure of the cytoplasmic membrane, and what...
Cell Structure and Function: 1. What is the basic structure of the cytoplasmic membrane, and what are its three critical roles in bacteria? (5) 2. What is the role of peptidoglycan in the cell, what are the two polysaccharides which make up the backbone, and what is the advantage for a cell lacking peptidoglycan? (4) 3. What is the difference between Gram-negative and Gram-positive Bacteria in the crosslinking of peptidoglycan? (2) 4. How and why does the Gram stain differentially...
2. Complete the following table by predicting colors of bacteria with- and without cell wall as...
2. Complete the following table by predicting colors of bacteria with- and without cell wall as they are processed through the steps of Gram staining. Steps of Gram Staining Bacteria containing thick cell wall Bacteria containing thin cell wall (LPS) Crystal violet treatment lodine Decolorization Safranin
2. Complete the following table by predicting colors of bacteria with- and without cell wall as...
2. Complete the following table by predicting colors of bacteria with- and without cell wall as they are processed through the steps of Gram staining. Steps of Gram Staining Bacteria containing thick cell Bacteria containing thin cell wall wall (LPS) Crystal violet treatment lodine Decolorization Safranin
need an explanation of how the gram stain is used and why it works. (this is...
need an explanation of how the gram stain is used and why it works. (this is not the gram stain procedure, but rather, how the dyes and steps work together to show Gram positive and Gram negative cell wall types)
Question 7 1.25 pts Crystal violet is used for? (choose all that apply) Stain to distinguish...
Question 7 1.25 pts Crystal violet is used for? (choose all that apply) Stain to distinguish Gram -/+ bacteria Disinfectant pH indicator Hair, fabric dye Ingredient in paints and ink Question 8 1.25 pts Indigo is used for (choose all that apply): Ingredient in paints Food coloring Fabric dye organic semiconductor
Which of the following has peptidoglycan as a major constituent of cell wall? A. Gram-negative bacteria...
Which of the following has peptidoglycan as a major constituent of cell wall? A. Gram-negative bacteria B. Gram-positive bacteria C. Fungi D. None of these
QUESTION 37 Which of these would be included in the Gram-positive cell envelope? Cytosol, cell wall,...
QUESTION 37 Which of these would be included in the Gram-positive cell envelope? Cytosol, cell wall, teichoic acid, LPS Peptidoglycan, slime-layer, cytosol, teichoic acid cytosol, peptidoglycan, te choic acid, capsule Plasma membrane, peptidoglycan, techoic acid, capsule Plasma membrane, cell walt, peptidoglycan, LPS
30. PRINCIPLES OF BIOLOGY BIOL 1456 Bacteria that retain crystal violet stain differ from those that do not retain the stain in having a a. thick lipoprotein layer. c. thicker peptidoglycan layer. b. thick lipopolysaccharide layer. o d cell wall.
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
Cell Structure and Function: 1. What is the basic structure of the cytoplasmic membrane, and what are its three critical roles in bacteria? (5) 2. What is the role of peptidoglycan in the cell, what are the two polysaccharides which make up the backbone, and what is the advantage for a cell lacking peptidoglycan? (4) 3. What is the difference between Gram-negative and Gram-positive Bacteria in the crosslinking of peptidoglycan? (2) 4. How and why does the Gram stain differentially...
2. Complete the following table by predicting colors of bacteria with- and without cell wall as they are processed through the steps of Gram staining. Steps of Gram Staining Bacteria containing thick cell wall Bacteria containing thin cell wall (LPS) Crystal violet treatment lodine Decolorization Safranin
2. Complete the following table by predicting colors of bacteria with- and without cell wall as they are processed through the steps of Gram staining. Steps of Gram Staining Bacteria containing thick cell Bacteria containing thin cell wall wall (LPS) Crystal violet treatment lodine Decolorization Safranin
Question 7 1.25 pts Crystal violet is used for? (choose all that apply) Stain to distinguish Gram -/+ bacteria Disinfectant pH indicator Hair, fabric dye Ingredient in paints and ink Question 8 1.25 pts Indigo is used for (choose all that apply): Ingredient in paints Food coloring Fabric dye organic semiconductor
QUESTION 37 Which of these would be included in the Gram-positive cell envelope? Cytosol, cell wall, teichoic acid, LPS Peptidoglycan, slime-layer, cytosol, teichoic acid cytosol, peptidoglycan, te choic acid, capsule Plasma membrane, peptidoglycan, techoic acid, capsule Plasma membrane, cell walt, peptidoglycan, LPS
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