need an explanation of how the gram stain is used and why it works. (this is not the gram stain procedure, but rather, how the dyes and steps work together to show Gram positive and Gram negative cell wall types)
The Gram staining technique is based on the characteristics of cell wall of bacteria. Gram positive bacteria have cell wall made of peptidoglycan while gram negative bacteria have cell wall made of lipids.
So, when crystal violet and iodine is added, crystal violet iodine complex forms which gets adhered to peptiglycan layer and so gram Positive bacteria will appear purple in color. When decoloriser by acid alcohol, this complex gets degraded in gram negative bacteria. So, when counter stain like saffronin is added, it takes up the color of counter stain and appears pink.
need an explanation of how the gram stain is used and why it works. (this is...
QUESTION 15 Why is crystal violet used in the Gram stain retained in the cell wall of Gram positive bacteria, but not in Gram negative bacteria? Gram positive bacteria have a thick peptidoglycan layer while Gram negative bacteria have a thin peptidoglycan layer. Gram positive bacteria form spores and Gram negative do not. O Gram positive bacteria have no peptidoglycan layer and Gram negative bacteria have a thick cell wall O Gram positive bacteria have capsules and Gram negative bacteria...
Why are basic dyes needed to stain bacteria? a. They are negative in charge b. Nigrosin is an example of a basic dye c. The cell wall of bacteria is positive in charge d. They are positive in charge e. They do not have a chromophore
why isn't the gram stain used on acid fast bacteria ?if you did Gram stain acid fast bacteria ,what would their Gram reaction be?what is the gram reaction of non acid fast bacteria
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be after a Gram-stain? What about Gram-negative cells? (2 pts.) a rum + - purple Gram -- pink 2. What characteristics can be determined in a Gram-stain? (2 pts) 3. List all of the things that can go happen during the Gram-staining process that could lead to an incorrect or poor result. (2pts.) 4. Why are direct gram stains ordered on clinical specimens? (2pts.) 5....
Lab Supplemental Questions hital Questions Acid-Fast Stain 1. Why is acid-alcohol used as the decoloriting agent in the dio Aud-alcohol is used as the decolorizing Staining because it ensures that the pro form non acld fast organisme. agent in the acid-fast stain? orizing agent in the acid-fast the primary stain is removed 2. Why don't we Gram stain the mycobacterium? stain both acid-fast and non-acid-fast bacteria stain red, what 3. If at the end of the acid-fast stain both acid-fast...
In the Gram Stain, safranin is the dye used to stain gram negative bacteria. This solution is 0.5%(wt/vol) safranin. How many milligrams of safranin are in a 120 mL safranin solution? Report your answer to two decimal places. Perform all calculations in a single step using Excel to avoid accruing rounding error.
The Gram stain, commonly used to classify a bacterial isolate into one of two groups, is based upon the characteristics of which of the following structures? the ribosomes the cell wall the flagella the genetic material
i need help with number 7 please
Questions: Yes or No: Is the Gram stain 100% accurate? 1. NO 2. Yes or No: Is the KOH test 100% accurate? NO 3. Yes or No: Is the vancomycin test 100 % accurate? NO 4. What cell structure causes the stringing in the KOH test? (a) the cell wall, (b) the lipopolysaccharide,(c) the DNA, (d) proteins, (e) mucus 5. Why is a Gram stain result important for treating physicians? (a) for prescribing...
4. Amelia performs a Gram stain of Staphylococcus aureus but forgets to add the iodine. How will her slide appear when she examines it under the microscope? Explain why. (15 points) 7. Give an example of an acidic dye. Where would this dye stain in relation to the cell? Why? (10 points) spina Eluid indian Ink can determine if a cell has a gelatinous capsule. 8. Roger prepares a simple stain of Streptococcus pyogenes using safranin, and a separate, Gram...
The negative stain is used to? a. determine Gram reaction b.visualize fimbriae c.determine cell size. d.visualize endospores. e.determine flagella arrangement.