Question

based on chromatographic methods lab which dye ( methylene blue and methylene orange) is more polor ?

how did you distinguish between chlorophyll a nad chlorophyll b in your TLC analysis ? which structural feature of the molecule accounts for this observation ?

compare and contrst your TLC results whe n100% acetone was used vs when 100% hexane was used what do your result suggest the polarities of the dyes in spinach ?

Answer 1

When we used polar solvent in chromatographic separation,polar compound will move faster then non-polar compound. In the separati on of m ethylene blue and methylene orange with polar solvent methylene orange moved faster and give spot near the solvent front. While methylene blue which is not polar, so it will not so much interact with solvent and not move so much. Methlene blue CH Methyl Orange GH ofs