Homework Answers
1. RNAse is a nuclease belonging to EC 2.7 and 3.1 for phosphorlytic and hydrolytic activities. it cleaves the phosphodiester bond in presence of water to liberate sugar molecule.
2. addition of OH group occurs on product. it comes from water molecule. two histidines in active site of enzyme are involved in transfer of Oh group as indicated by arrows in the figure.
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The following questions are related to enzyme mechanisms and are based on the mechanism of RNase...
6. Free Energy Calculation (10 pts) Ribonuclease-A (RNase-A), a digestive enzyme hydrolyzes RNA to its component...
6. Free Energy Calculation (10 pts) Ribonuclease-A (RNase-A), a digestive enzyme hydrolyzes RNA to its component nucleotides. An inhibitor of the enzyme, 2'-cyclic monophosphate, can be used to study the enthalpy of nucleotide binding to RNase-A. The scheme showing below is based on the isolation of 2',3'-cyclic nucleotides from RNase A digests of RNA. There are four types of 2,3'-cyclic nucleotides. RNase-A hydrolysis of one of them, cytidine 2',3'-cyclic phosphate, has been studied extensively. The reaction is given as: cytidine...
48. Use the figure below to answer the following questions. TS.Cyelle nucleotide RNA 0 0 B...
48. Use the figure below to answer the following questions. TS.Cyelle nucleotide RNA 0 0 B O-POCHO 0 Н M 31 H NIE 0 HO 1 NH -0 H0 CH Ване ANI OPO H His 119 His 119 он -O-P- CH, 0-11 OPO 0- His 119 a. In the first step of the reaction. His 12 is acting as a and His 119 is acting as a a. General base, general acid b. General acid, general base c. Nucleophile, electrophile...
2) (14 pts) Shown on the next page is a schematic drawing of the mechanism for...
2) (14 pts) Shown on the next page is a schematic drawing of the mechanism for an RNase. Residues that are important for catalysis are also shown schematically. (a) Refer to the scheme and describe the roles of His 12, Lys 41, and His 119 in the catalytic mechanism. List all of the following that apply: general acid/base catalysis (GABC), covalent catalysis, electrostatic stabilization of transition state. For a GABC, indicate in which step(s) of the mechanism the residue acts...
2. The reaction below is catalyzed by the CK enzyme: NH2 OP H2N H₂N N Ń...
2. The reaction below is catalyzed by the CK enzyme: NH2 OP H2N H₂N N Ń cooK COO - INN CO + ATP + ADP Below is a picture of the active site for this enzyme. The red atoms are the amino acids in the CK protein. The blue structure is the substrate, the orange is the phosphate end of ATP. All dashed lines are showing interactions and the curved arrows are showing the mechanism. The wavy lines indicate that...
2. The reaction below is catalyzed by the CK enzyme: NH O | NHA | СК...
2. The reaction below is catalyzed by the CK enzyme: NH O | NHA | СК O= H₂N Ņ coo eo T N con + ATP + ADP Below is a picture of the active site for this enzyme. The red atoms are the amino acids in the CK protein. The blue structure is the substrate, the orange is the phosphate end of ATP. All dashed lines are showing interactions and the curved arrows are showing the mechanism. The wavy...
Jame: . Complete this mechanism for the following substitution reaction. Note: this reaction is balanced. H-Br...
Jame: . Complete this mechanism for the following substitution reaction. Note: this reaction is balanced. H-Br Н,0 OH -CH3 H20 Step 1: proton transfer Step 2: lose water Step 3: attack the carbocation Br -CH3 . Each reaction above is reversible. In question 1, what effect would adding more HBr have on the equilibrium? Would there be more or less alkyl bromide product present at equilibrium? . Two dehydrations can occur on glycerol, the principle carrier molecule in vaping devices....
MECHANISMS (6 pts.) 4. Choose one (1] of the following: Write a complete, detailed mechanism for...
MECHANISMS (6 pts.) 4. Choose one (1] of the following: Write a complete, detailed mechanism for the following reaction: a. CHOH, H; он When dissolved in water, trichloroacetaldehyde (chloral) exists primarily as chloral hydrate ("knockout drops"), a geminal diol. Show the structure of the product and write the mechanism for the formation of chloral hydrate from chloral. b. Cl O Cl When cyclohexanone is heated in the presence of a large amount of acetone, cyanohydrins, and a small amount of...
Mechanisms (40 marks) 1 Draw the mechanism of each of the following reactions. (5 marks cach)...
Mechanisms (40 marks) 1 Draw the mechanism of each of the following reactions. (5 marks cach) (a) HCHO + H HO H- OH OH CCI,CHO +H30 Bocco HO OH , c=NR + H2O c=0 + HN-R- 1. LDA 2. CH31 CHI 2-methyl-3-Pentanone 3-Pentanone Treat LDA as B OH но 2 HẠC-CHO H 2-Hydroxybutanal B-hydroxy carbonyl L a .B-unsaturated carbonyl
please help with question 5 and question 6. thank you 12] QUESTIONS Which of the following...
please help with question 5 and question 6. thank you
12] QUESTIONS Which of the following shows the correct mechanism of the reaction depicted below? Come on 02-0 heyran (2) NH, Na+ CE- A B C Mechanism (1) Mechanism (2) Both mechanisms are correct, the reaction occurs with inversion of stereochemistry, as expected of S2 reactions None of the mechanisms are correct, the competing elimination reaction is not shown. D [2] QUESTION 6 Which of the following reactions is in...
Identify the mechanism by which each of the reactions above proceeds from among the mechanisms listed....
Identify the mechanism by which each of the reactions above
proceeds from among the mechanisms listed. Use the letters
a - i for your answers.
Methanol to + + NaOCH3 NOCH Mothands t º + HOCH • NAGI HOCHZ + NaCl OH NaHCO3 o + H2O + CO2 + Nal a = Proton transfer b = Lewis acid base c = Radical chain substitution d=Radical chain addition e = Electrophilic addition f=E1 Elimination g=E2 Elimination h=Syl Nucleophilic substitution i= Sy2...
6. Free Energy Calculation (10 pts) Ribonuclease-A (RNase-A), a digestive enzyme hydrolyzes RNA to its component nucleotides. An inhibitor of the enzyme, 2'-cyclic monophosphate, can be used to study the enthalpy of nucleotide binding to RNase-A. The scheme showing below is based on the isolation of 2',3'-cyclic nucleotides from RNase A digests of RNA. There are four types of 2,3'-cyclic nucleotides. RNase-A hydrolysis of one of them, cytidine 2',3'-cyclic phosphate, has been studied extensively. The reaction is given as: cytidine...
48. Use the figure below to answer the following questions. TS.Cyelle nucleotide RNA 0 0 B O-POCHO 0 Н M 31 H NIE 0 HO 1 NH -0 H0 CH Ване ANI OPO H His 119 His 119 он -O-P- CH, 0-11 OPO 0- His 119 a. In the first step of the reaction. His 12 is acting as a and His 119 is acting as a a. General base, general acid b. General acid, general base c. Nucleophile, electrophile...
2) (14 pts) Shown on the next page is a schematic drawing of the mechanism for an RNase. Residues that are important for catalysis are also shown schematically. (a) Refer to the scheme and describe the roles of His 12, Lys 41, and His 119 in the catalytic mechanism. List all of the following that apply: general acid/base catalysis (GABC), covalent catalysis, electrostatic stabilization of transition state. For a GABC, indicate in which step(s) of the mechanism the residue acts...
2. The reaction below is catalyzed by the CK enzyme: NH2 OP H2N H₂N N Ń cooK COO - INN CO + ATP + ADP Below is a picture of the active site for this enzyme. The red atoms are the amino acids in the CK protein. The blue structure is the substrate, the orange is the phosphate end of ATP. All dashed lines are showing interactions and the curved arrows are showing the mechanism. The wavy lines indicate that...
2. The reaction below is catalyzed by the CK enzyme: NH O | NHA | СК O= H₂N Ņ coo eo T N con + ATP + ADP Below is a picture of the active site for this enzyme. The red atoms are the amino acids in the CK protein. The blue structure is the substrate, the orange is the phosphate end of ATP. All dashed lines are showing interactions and the curved arrows are showing the mechanism. The wavy...
Jame: . Complete this mechanism for the following substitution reaction. Note: this reaction is balanced. H-Br Н,0 OH -CH3 H20 Step 1: proton transfer Step 2: lose water Step 3: attack the carbocation Br -CH3 . Each reaction above is reversible. In question 1, what effect would adding more HBr have on the equilibrium? Would there be more or less alkyl bromide product present at equilibrium? . Two dehydrations can occur on glycerol, the principle carrier molecule in vaping devices....
MECHANISMS (6 pts.) 4. Choose one (1] of the following: Write a complete, detailed mechanism for the following reaction: a. CHOH, H; он When dissolved in water, trichloroacetaldehyde (chloral) exists primarily as chloral hydrate ("knockout drops"), a geminal diol. Show the structure of the product and write the mechanism for the formation of chloral hydrate from chloral. b. Cl O Cl When cyclohexanone is heated in the presence of a large amount of acetone, cyanohydrins, and a small amount of...
Mechanisms (40 marks) 1 Draw the mechanism of each of the following reactions. (5 marks cach) (a) HCHO + H HO H- OH OH CCI,CHO +H30 Bocco HO OH , c=NR + H2O c=0 + HN-R- 1. LDA 2. CH31 CHI 2-methyl-3-Pentanone 3-Pentanone Treat LDA as B OH но 2 HẠC-CHO H 2-Hydroxybutanal B-hydroxy carbonyl L a .B-unsaturated carbonyl
please help with question 5 and question 6. thank you
12] QUESTIONS Which of the following shows the correct mechanism of the reaction depicted below? Come on 02-0 heyran (2) NH, Na+ CE- A B C Mechanism (1) Mechanism (2) Both mechanisms are correct, the reaction occurs with inversion of stereochemistry, as expected of S2 reactions None of the mechanisms are correct, the competing elimination reaction is not shown. D [2] QUESTION 6 Which of the following reactions is in...
Identify the mechanism by which each of the reactions above
proceeds from among the mechanisms listed. Use the letters
a - i for your answers.
Methanol to + + NaOCH3 NOCH Mothands t º + HOCH • NAGI HOCHZ + NaCl OH NaHCO3 o + H2O + CO2 + Nal a = Proton transfer b = Lewis acid base c = Radical chain substitution d=Radical chain addition e = Electrophilic addition f=E1 Elimination g=E2 Elimination h=Syl Nucleophilic substitution i= Sy2...
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